Multifunctional N, Fe-doped carbon dots with peroxidase-like activity for the determination of H2O2 and ascorbic acid and cell protection against oxidation

被引：6

作者：

Chen, Shenna ^{[1
]}

Li, Ronghui ^{[1
]}

Zhao, Bo ^{[2
]}

Fang, Mei ^{[1
]}

Tian, Yun ^{[3
]}

Lei, Yuhua ^{[3
]}

Li, Yayong ^{[4
]}

Geng, Lina ^{[1
]}

机构：

[1] Hebei Normal Univ, Coll Chem & Mat Sci, Hebei Technol Innovat Ctr Energy Convers Mat & Dev, Shijiazhuang 050024, Peoples R China

[2] Hebei Med Univ, Expt Ctr Teaching, Shijiazhuang 050017, Peoples R China

[3] Hebei Med Univ, Coll Basic Med, Shijiazhuang 050017, Peoples R China

[4] Shijiazhuang Peoples Hosp, Dept Rehabil Med, Shijiazhuang 050000, Peoples R China

来源：

MICROCHIMICA ACTA | 2024年 / 191卷 / 07期

基金：

中国国家自然科学基金;

关键词：

N; Fe-codoped carbon dots; Peroxidase-like activity; Colorimetric detection; H2O2; Ascorbic acid; HYDROGEN-PEROXIDE; QUANTUM DOTS; COLORIMETRIC DETECTION; NANOPARTICLES;

D O I：

10.1007/s00604-024-06456-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Multifunctional N, Fe-doped carbon dots (N, Fe-CDs) were synthesized by the one-step hydrothermal method using ferric ammonium citrate and dicyandiamide as raw materials. The N, Fe-CDs exhibited peroxidase-like (POD) activity by catalyzing the oxidization of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to the green oxidation state ox-TMB in the presence of hydrogen peroxide (H2O2). Subsequently, based on the POD activity of N, Fe-CDs, an efficient and sensitive colorimetric method for the detection of H2O2 and ascorbic acid (AA) was established with a limit of detection of 0.40 mu M and 2.05 mu M. The proposed detection method has been successfully applied to detect AA in fruit juice, vitamin C tablets, and human serum samples and has exhibited excellent application prospects in biotechnology and food fields. Furthermore, N, Fe-CDs also showed a protective effect on the cell damage caused by H2O2 and could be used as an antioxidant agent.

引用

页数：10

共 25 条

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