The unfixed light pattern contributes to depressive-like behaviors in male mice

被引：2
作者：
Liu Q. [1 ,2 ]
Meng Q. [1 ,2 ]
Ding Y. [1 ,2 ]
Jiang J. [1 ,2 ]
Kang C. [1 ,2 ]
Yuan L. [1 ,2 ]
Guo W. [1 ,2 ]
Zhao Z. [1 ,2 ]
Yuan Y. [1 ,2 ]
Wei X. [1 ,2 ]
Hao W. [1 ,2 ]
机构：
[1] Department of Toxicology, School of Public Health, Peking University, Beijing
[2] Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing
来源：
Chemosphere | 2023年 / 339卷
关键词：
Circadian disruptions; Depression; Light pollution; Orexin A; Rotating shift work; Suprachiasmatic nucleus;
D O I：
10.1016/j.chemosphere.2023.139680
中图分类号：
学科分类号：
摘要：
Light pollution is now associated with an increased incidence of mental disorders in humans, and the unfixed light pattern (ULP) is a common light pollution that occurs in such as rotating shift work. However, how much contribution the ULP has to depression and its potential mechanism are yet unknown. Our study aimed to investigate the effect of the ULP on depressive-like behaviors in mice and to explore the links to the circadian-orexinergic system. Male C57BL/6 J mice were exposed to the ULP by subjecting them to an alternating light pattern every 6 days for 54 days. The tail suspension test (TST) and forced swimming test (FST) were conducted to assess depressive-like behaviors. The rhythm of locomotor activity and the circadian expression of cFOS in the suprachiasmatic nucleus (SCN), clock genes in the liver, and corticosterone (CORT) in serum were detected to observe changes in the circadian system. The circadian expression of orexin-A (OX-A) in the lateral hypothalamus area (LHA) and dorsal raphe nucleus (DRN) and serotonin (5-HT) in the DRN were measured to determine alterations in the orexinergic system. The results showed that mice exposed to the ULP exhibited increased immobility time in the TST and FST. The ULP significantly disrupted the circadian rhythm of locomotor activity, clock genes in the liver, and CORT in the serum. Importantly, when exposed to the ULP, cFOS expression in the SCN showed decreased amplitude. Its projection area, the LHA, had a lower mesor of OX-A expression. OX-A projection to the DRN and 5-HT expression in the DRN were reduced in mesor. Our research suggests that the ULP contributes to depressive-like behaviors in mice, which might be related to the reduced amplitude of circadian oscillation in the SCN and hypoactivity of the orexinergic system. These findings may provide novel insights into rotating shift work-related depression. © 2023
引用
收藏
相关论文
共 92 条
[51]  
Li S.-X., Liu L.-J., Xu L.-Z., Gao L., Wang X.-F., Zhang J.-T., Lu L., Diurnal alterations in circadian genes and peptides in major depressive disorder before and after escitalopram treatment, Psychoneuroendocrinology, 38, pp. 2789-2799, (2013)
[52]  
Livak K.J., Schmittgen T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method, Methods, 25, pp. 402-408, (2001)
[53]  
Luo D.D., An S.C., Zhang X., Involvement of hippocampal serotonin and neuropeptide Y in depression induced by chronic unpredicted mild stress, Brain Res. Bull., 77, pp. 8-12, (2008)
[54]  
Marcus J.N., Aschkenasi C.J., Lee C.E., Chemelli R.M., Saper C.B., Yanagisawa M., Elmquist J.K., Differential expression of orexin receptors 1 and 2 in the rat brain, J. Comp. Neurol., 435, pp. 6-25, (2001)
[55]  
Marston O.J., Williams R.H., Canal M.M., Samuels R.E., Upton N., Piggins H.D., Circadian and dark-pulse activation of orexin/hypocretin neurons, Mol. Brain, 1, (2008)
[56]  
Maruani J., Geoffroy P.A., Multi-level processes and retina-brain pathways of photic regulation of mood, J. Clin. Med., 11, (2022)
[57]  
Michelsen K.A., Schmitz C., Steinbusch H.W.M., The dorsal raphe nucleus - from silver stainings to a role in depression, Brain Res. Rev., 55, pp. 329-342, (2007)
[58]  
Minami Y., Yoshikawa T., Nagano M., Koinuma S., Morimoto T., Fujioka A., Furukawa K., Ikegami K., Tatemizo A., Egawa K., Tamaru T., Taniguchi T., Shigeyoshi Y., Transgenic rats expressing dominant negative BMAL1 showed circadian clock amplitude reduction and rapid recovery from jet lag, Eur. J. Neurosci., 53, pp. 1783-1793, (2021)
[59]  
Moralia M.-A., Quignon C., Simonneaux M., Simonneaux V., Environmental disruption of reproductive rhythms, Front. Neuroendocrinol., 66, (2022)
[60]  
Muscogiuri G., Poggiogalle E., Barrea L., Tarsitano M.G., Garifalos F., Liccardi A., Pugliese G., Savastano S., Colao A., Obesity Progra nutrit E., Exposure to artificial light at night: a common link for obesity and cancer, Eur. J. Cancer, 173, pp. 263-275, (2022)
12345678910