Highly Sensitive Colorimetric Detection of Listeria monocytogenes by Using Gold Nanoparticles Based on Magnetic Metal-Organic Framework Separation

被引：0

作者：

Du J. ^{[1
]}

Chen X. ^{[1
]}

Liu K. ^{[1
]}

Liu J. ^{[1
]}

Luo P. ^{[2
]}

Liang D. ^{[3
]}

Bai Y. ^{[1
]}

机构：

[1] College of Food and Bioengineering, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou

[2] NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing

[3] Applied Nutrition I, China National Center for Food Safety Risk Assessment, Beijing

来源：

Shipin Kexue/Food Science | 2023年 / 44卷 / 05期

关键词：

colorimetric assay; gold nanoparticles; Listeria monocytogenes; magnetic separation;

D O I：

10.7506/spkx1002-6630-20220316-179

中图分类号：

学科分类号：

摘要：

In this study, magnetic metal organic framework composites as capture probe and immunofunctionalized gold nanoparticles as signal probe were used to develop a rapid colorimetric method for the detection of Listeria monocytogenes. Under optimal conditions, the visual detection limit for L. monocytogenes was 1.2 × 103 CFU/mL. The results of ultraviolet spectroscopy showed a good linear relationship in the range of 1.2 × 101‒1.2 × 108 CFU/mL (Y = 0.519 ‒ 0.043X, R2 = 0.978), the detection limit was as low as 0.45 CFU/mL, and the intra-batch coefficient of variation of all concentration gradients was between 0.31% and 1.30%. The recoveries for spiked chicken breast were 91.1%‒108.7% with coefficient of variation less than 7.4%, and the result was consistent with that of the plate counting method. In conclusion, the proposed assay is rapid, accurate sensitive, and highly applicable to the detection of foodborne pathogens. © 2023 Chinese Chamber of Commerce. All rights reserved.

引用

页码：360 / 367

页数：7

共 33 条

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