Regulation of Quorum Sensing on Biofilm Formation and Swimming Motility in Hafnia alvei

被引：1
作者：
Zhu Y. [1 ,2 ,3 ]
Hou H. [1 ,3 ]
Zhang G. [1 ,3 ]
Hao H. [3 ]
机构：
[1] School of Food Science and Technology, Dalian Polytechnic University, Dalian
[2] School of Food and Drug, Luoyang Normal University, Luoyang
[3] Liaoning Key Lab for Aquatic Processing Quality and Safety, Dalian
来源：
Shipin Kexue/Food Science | 2020年 / 41卷 / 14期
关键词：
Biofilm formation; Hafnia alvei; Quorum sensing; Swimming motility;
D O I：
10.7506/spkx1002-6630-20190624-288
中图分类号：
学科分类号：
摘要：
The aim of this study was to evaluate the regulation of quorum sensing on biofilm formation and swimming motility of Hafnia alvei H4 isolated from spoiled instant sea cucumber. Both the luxR and luxI genes were knocked out to construct the mutant strain ΔluxRI. Crystal violet staining method was used to measure the biofilm formation of H. alvei H4, and the structure of the biofilm was observed by scanning electron microscopy (SEM). In addition, the spread plate assay was used to detect swimming motility. The relative expression levels of biofilm- and motility-related genes (f l gA, flgE, fliA, flhD and flhC) were measured by real-time PCR method. Results showed that growth of H. alvei H4 was not affected by depletion of the luxRI genes. However, AHLs production was found deficient in the ΔluxRI strain. In addition, biofilm formation of ΔluxRI was significantly inhibited (P < 0.05), and fewer cells were observed compared with the wild-type strain by SEM on the medium, and fewer reticular structures were formed by exopolysaccharides (EPS) from ΔluxRI. Furthermore, swimming motility of ΔluxRI was found to be obviously decreased. The relative expression levels of the flgA, flgE, fliA, flhD and flhC genes were significantly (P < 0.05) reduced. Therefore, a conclusion can be drawn that biofilm formation and swimming motility of H. alvei H4 is regulated by quorum sensing. © 2020, China Food Publishing Company. All right reserved.
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页码：169 / 174
页数：5
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