The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia

被引：1

作者：

Myhrer, Dina-Marie Munkelien ^{[1
]}

Froystad, Monica ^{[1
,2
]}

Roland, Marie Cecilie Paasche ^{[3
,4
]}

Ueland, Thor ^{[1
,2
,5
]}

Lekva, Tove ^{[2
,6
]}

机构：

[1] Univ Oslo, Fac Med, Oslo, Norway

[2] Oslo Univ Hosp, Res Inst Internal Med, Oslo, Norway

[3] Oslo Univ Hosp, Dept Obstet, Oslo, Norway

[4] Oslo Univ Hosp, Dept Med Biochem, Oslo, Norway

[5] Univ Hosp North Norway, Thrombosis Res Ctr TREC, Div Internal Med, Tromso, Norway

[6] Oslo Univ Hosp, Rikshosp, Res Inst Internal Med, Sognsvannsveien 20, N-0027 Oslo, Norway

来源：

BIOLOGY OF REPRODUCTION | 2024年 / 111卷 / 02期

关键词：

pre-eclampsia; RPPH1; lncRNA; PROLIFERATION; RNA; EXPRESSION; MIGRATION; LNCRNAS;

D O I：

10.1093/biolre/ioae069

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Previous studies show differentially expressed long non-coding RNA present in the placenta from women with pre-eclampsia, potentially playing a vital role in the pathogenesis of the complication. In a published microarray study, Ribonuclease P RNA component H1 was decreased in leukocytes from women that later developed pre-eclampsia. We hypothesized that Ribonuclease P RNA component H1 decreased during pregnancy in women developing pre-eclampsia and important for the development of the complication. We isolated RNA from extracellular vesicles, leukocytes and plasma using blood samples taken at weeks 22-24 and 36-38 in women who subsequently developed pre-eclampsia and from healthy pregnancy. The expression of Ribonuclease P RNA component H1 was quantified using qPCR. Expression of Ribonuclease P RNA component H1 at 22-24 weeks was further examined to investigate its discriminatory potential of subsequent pre-eclampsia and association with clinical markers. We found lower expression of Ribonuclease P RNA component H1 in leukocytes at 22-24 and 36-38 weeks amongst women who subsequent developed pre-eclampsia compared with those who did not, while increased Ribonuclease P RNA component H1 expression was found in plasma at 36-38 weeks. Pre-eclampsia risk factors could not account for this difference in the Ribonuclease P RNA component H1 expression. Prediction of pre-eclampsia at 22-24 weeks using Ribonuclease P RNA component H1 expression in leukocytes in addition to the screening algorithm used today had a significantly better performance. In conclusion, Ribonuclease P RNA component H1 expression in leukocytes was significantly decreased in women with pre-eclampsia, and the expression at 22-24 weeks associated with the subsequent development of pre-eclampsia. Ribonuclease P RNA component H1 in leukocytes may be a useful biomarker for prediction and/or early detection of pre-eclampsia and an unknown regulator of the signaling affecting immune cells.

引用

页码：427 / 435

页数：9