Overexpression of Discoidin Domain Receptor 1 Is Associated with Tumor Size in Esophageal Squamous Cell Carcinoma

被引：0
作者：
Afsaneh Shafiei [1 ,2 ]
Jafari S.M. [1 ,2 ]
Jazi M.S. [1 ]
Pourjam M. [1 ,2 ]
Asadi J. [1 ,2 ]
机构：
[1] Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan
[2] Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan
来源：
Cell and Tissue Biology | 2024年 / 18卷 / 3期
关键词：
biomarker; DDR1; esophageal squamous cell carcinoma; tyrosine kinase receptor;
D O I：
10.1134/S1990519X24700226
中图分类号：
学科分类号：
摘要：
Abstract: Background. Discoidin domain receptor 1 (DDR1) is confirmed as a member of the transmembrane receptor tyrosine kinase (RTK) superfamily, which plays an influential role in various cancers. Increasing evidence has suggested that DDR1 is plays role in cancer progression and metastasis. However, DDR1 function and the underlying mechanism of DDR1 receptor signaling pathways is largely unknown, especially in esophageal squamous cell carcinoma (ESCC). Methods and results. We obtained 42 paired samples of ESCC tumors (N = 21) and adjacent normal tissues (N = 21) from newly diagnosed ESCC patients. In this study, the expression of DDR1 in esophageal cancer was retrieved based on the GENT2, OncoDB databases. AlsoqRT-PCR experiments was used to measure DDR1 expression experimentally in ESCC tissues compared to adjacent non-tumor tissues (NTs). Moreover, the correlation between the expression of DDR1 and clinic pathologic factors was analyzed. The diagnostic significance of the results was subsequently evaluated utilizing the receiver operating characteristic (ROC) curve. The result showed that mRNA expression of DDR1 was up-regulated in ESCC tissues compared with NTs (p = 0.0001). Statistical analysis revealed that DDR1 expression was significantly higher in samples with a tumor size of more than five centimeters (p = 0.02). The ROC curve analysis indicated that DDR1 expression level in tissue potentially have high accuracy for diagnosing ESCC compared with adjacent non-tumor tissues NTs (AUC = 0.92, Specificity = 84%, sensitivity = 96%). Conclusions. Our findings suggest that overexpression of the DDR1 might play a function in promoting cancer and tumor size. Moreover, DDR1 can potentially act as a novel biomarker for identifying ESCC patients, indicating the potential of this receptor for ESCC-targeted therapeutics. © Pleiades Publishing, Ltd. 2024. ISSN 1990-519X, Cell and Tissue Biology, 2024, Vol. 18, No. 3, pp. 289–295. Pleiades Publishing, Ltd., 2024.
引用
收藏
页码：289 / 295
页数：6
相关论文
共 22 条
[1]  
Alves F., Vogel W., Mossie K., Millauer B., Hofler H., Ullrich A., Distinct structural characteristics of discoidin I subfamily receptor tyrosine kinases and complementary expression in human cancer, Oncogene, 10, (1995)
[2]  
Borza C.M., Pozzi A., Discoidin domain receptors in disease, Matrix Biol, 34, (2014)
[3]  
Castro-Sanchez L., Soto-Guzman A., Guaderrama-Diaz M., Cortes-Reynosa P., Salazar E.P., Role of DDR1 in the gelatinases secretion induced by native type IV collagen in MDA-MB-231 breast cancer cells, Clin. Exp. Metastasis, 28, (2011)
[4]  
Chou S.T., Peng H.-Y., Mo K.-C., Hsu Y.-M., Wu G.-H., Hsiao J.-R., Lin S.-F., Wang H.-D., Shiah S.-G., MicroRNA-486-3p functions as a tumor suppressor in oral cancer by targeting DDR1, J. Exp. Clin, 38, (2019)
[5]  
Chua H.-H., Yeh T.-H., Wang Y.-P., Huang Y.-T., Sheen T.-S., Lo Y.-C., Chou Y.-C., Tsai C.-H., Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma, HeadNeck, 30, (2008)
[6]  
Ford C.E., Lau S.K., Zhu C.Q., Andersson T., Tsao M.S., Vogel W.F., Expression and mutation analysis of the discoidin domain receptors 1 and 2 in non-small cell lung carcinoma, Br. J. Cancer, 96, (2007)
[7]  
Hu X., Wu D., He X., Zhao H., He Z., Lin J., Wang K., Wang W., Pan Z., Lin H., circGSK3β promotes metastasis in esophageal squamous cell carcinoma by augmenting β-catenin signaling, Mol. Cancer, 18, (2019)
[8]  
Lemmon M.A., Schlessinger J., Cell signaling by receptor tyrosine kinases, Cell, 141, (2010)
[9]  
Miao L., Zhu S., Wang Y., Li Y., Ding J., Dai J., Cai H., Zhang D., Song Y., Discoidin domain receptor 1 is associated with poor prognosis of non-small cell lung cancer and promotes cell invasion via epithelial-to-mesenchymal transition, Med. Oncol, 30, (2013)
[10]  
Oda K., Matsuoka Y., Funahashi A., Kitano H., A comprehensive pathway map of epidermal growth factor receptor signaling, Mol. Syst. Biol, 1, (2005)
123