Preparation of Glycerides Rich in n-3 PUFAs by Lipase-catalyzed Alcoholysis

n-3 PUFAs have various physiological activities, but their contents in natural oils are usually low. This study investigated the preparation of glycerides rich in n-3 PUFAs by Eversa Transform 2.0 (ET 2.0)-catalyzed alcoholysis of fish oil. The results showed that the ET 2.0 from Aspergillus oryzae could effectively identify n-3 PUFAs during alcoholysis. Saturated fatty acids and monounsaturated fatty acids were released from fish oil by ET 2.0-catalyzed alcoholysis, causing the production of n-3 PUFA-enriched glycerides. Under optimal conditions (1.6:1 of mass ratio of methanol to algal oil, 40% methanol aqueous solution, 4% ET 2.0 dosage, 35 "C, 2 h), the n-3 PUFA content in glyceride increased from the initial 39.88% to 67.87%, the content of EPA and DHA increased from 36.61% to 65.22%, and the yield of n-3 PUFAs was 56.33%. When tuna oil and algal oil were used as substrate, the content of n-3 PUFAs in tuna oil glycerides increased from 38.81% to 61.35%, and the content of n-3 PUFAs in algae oil glycerides increased from 45.96% rose to 64.09%. The n-3 PUFAs-enriched glycerides obtained by the above method have great application potential in the food and pharmaceutical industries. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.