Quantitative immunochromatographic assay for rapid and cost-effective on-site detection of benzo[a]pyrene in oilfield chemicals

被引：23

作者：

Li, Jinyan ^{[1
,2
,3
]}

Jiang, Luming ^{[4
,5
]}

Shu, Yong ^{[4
,5
]}

Song, Shanshan ^{[1
,2
,3
]}

Xu, Liguang ^{[1
,2
,3
]}

Kuang, Hua ^{[1
,2
,3
]}

Xu, Chuanlai ^{[1
,2
,3
]}

Guo, Lingling ^{[1
,2
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Resources, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Jiangsu, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[4] PetroChina Res Inst Petr Explorat & Dev, Beijing 10083, Peoples R China

[5] CNPC, Key Lab Oilfield Chem, Beijing 100083, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2024年 / 469卷

关键词：

Benzo[a]pyrene; Matrix-insensitive monoclonal antibody; Immunochromatographic strip assay; Oilfield chemicals; IMMUNOASSAY; BENZO(A)PYRENE; SENSOR;

D O I：

10.1016/j.jhazmat.2024.134100

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Contamination of oilfield chemicals (OFCs) by benzo[a]pyrene (B[a]P) is increasingly becoming a severe environmental security issue. There is an urgent need to develop a rapid and accurate method for B[a]P detection in OFCs. In this study, B[a]P hapten was designed using computer aided molecular design. A high -affinity, specific, and matrix -insensitive monoclonal antibody (mAb) with IC 50 values of 6.77 ng/mL was obtained. Based on this mAb, we developed a rapid gold nanoparticle-based immunochromatographic strip assay (GICA) with double Tline mode for on -site detection of B[a]P in OFCs samples. The GICA exhibited excellent detection performance in OFCs samples with strong acidity, strong alkalinity, and deep color. Under optimal conditions, the proposed method detected B[a]P in OFCs at 0.42 -300 mg/kg, and limit of detection was 0.23 -1.07 mg/kg. The recovery rate was 88 -106% with a coefficient of variation of 1.46 -6.35%. Confirmed by natural positive OFCs samples and high-performance liquid chromatography, this GICA is accurate and reliable, with great potential for rapid and cost-effective on -site detection.

引用

页数：11