Synthesis, characterization, antimicrobial and anti-biofilm studies of Ni(II) complex with Schiff base derived from 2-thiophene carboxaldehyde and phenyl acetic hydrazide

Schiff base titled (Z)-2-phenyl-N'-(thiophen-2-ylmethylene)acetohydrazide (HL) and its Ni (II) complex were obtained by the condensation of 2-thiophene carboxaldehyde and phenyl acetic hydrazide. The ligand (HL) was characterized using elemental analysis, Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1HNMR ), Ultraviolet-visible (UV-Vis.) spectroscopy, and Liquid chromatography-mass spectrometry (LC/MS) methods. Elemental analysis, FTIR, UV-Vis, and X-ray single crystal studies were used to elucidate the structure of the [NiL2] complex. The brown single crystal structure of the Ni(II) complex was elucidated by X-ray diffraction technique, and the complex structure was found to crystallize in a triclinic crystal system with P1 space group. With symmetries of 1-x, 1-y, and -z and -x, 1-y, and 1-z, the nickel atoms in the asymmetric unit are positioned on an inversion centre. It was determined that the antimicrobial activity of the newly synthesized HL and [NiL2] compounds was high on clinically important strains and had low minimal inhibitory concentration (MIC) values. In addition, the anti -biofilm activity of the compounds showed 45.14 % and 42.28 % biofilm inhibition, respectively, on the E. coli ATCC 25,922 strain at a concentration of 100 mu g/ml, whereas it showed 56.36 % and 42.28 % inhibition, respectively, on the P. aeruginosa ATCC 278533 strain showed an inhibitory effect.