Extraction, Purification and Structure Analysis of Soluble Acidic Polysaccharides from Soybean Dregs

被引：1
|
作者：
Wang S. [1 ]
Hu Y. [1 ]
Zhai L. [1 ]
Shi Z. [1 ]
Zhao J. [1 ]
机构：
[1] College of Food Science and Engineering, Changchun University, Changchun
来源：
Shipin Kexue/Food Science | 2021年 / 42卷 / 10期
关键词：
Separation and purification; Soluble acid sugar; Soybean okara; Structure analysis;
D O I：
10.7506/spkx1002-6630-20210118-187
中图分类号：
学科分类号：
摘要：
The crude polysaccharide extracted from soybean dregs was purified and fractionated into two soluble acid polysaccharide fractions with uniform charge, SSPS-A1 and SSPS-A2. SSPS-A1 was further subjected to gel filtration chromatography, yielding a homogenous polysaccharide with uniform charge, SSPS-A1-c. Its molecular mass and monosaccharide composition were measured, and its structure was analyzed by infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The results showed that SSPS-A1-c had a molecular mass of 31.6 kDa, mainly composed of galacturonic acid (GalA), galactose (Gal) and arabinose (Ara) with a molar ratio of 17.3:44.9:19.1 together with lesser amounts of rhamnose (Rha), glucose (Glc) and xylose (Xyl). IR spectroscopy analysis showed that SSPS-A1-c had the characteristic absorption peaks of acidic polysaccharides such as O-H, C-H and C=O and it may be methylated or acetylated to a certain extent. NMR results showed that SSPS-A1-c had chemical shifts of α-1, 5-Araf, α-t-Araf and β-t-Galp, which was speculated to contain arabinogalactan (AG)-type pectin domain. Besides, it also exhibited chemical shift of α-1, 4-GalpA, which was inferred to contain the homogalacturonan (HG)-type pectin domain. The NMR results were consistent with the monosaccharide composition and infrared analysis results. The research results will provide a theoretical basis for the application of soluble acidic polysaccharides from soybean dregs, and will be of great significance to the comprehensive development and utilization of soybean dregs. © 2021, China Food Publishing Company. All right reserved.
引用
收藏
页码：52 / 57
页数：5
相关论文
共 23 条
  • [1] CHEN F, HUANG G L., Antioxidant activity of polysaccharides from different sources of ginseng, International Journal of Biological Macromolecules, 125, pp. 906-908, (2019)
  • [2] XIAO H, FU X, CAO C L, Et al., Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum, International Journal of Biological Macromolecules, 121, pp. 407-414, (2019)
  • [3] ZHENG Y, BAI L, ZHOU Y P, Et al., Polysaccharides from Chinese herbal medicine for anti-diabetes recent advances, International Journal of Biological Macromolecules, 121, pp. 1240-1253, (2019)
  • [4] YANG L N, ZHANG H Y, ZHAO Y F, Et al., Chemical structure, chain conformation and rheological properties of pectic polysaccharides from soy hulls, International Journal of Biological Macromolecules, 148, pp. 41-48, (2020)
  • [5] NAKAMURA A, FUTUTA H, MAEDA H, Et al., Structural studies by stepwise enzymatic degradation of the main backbone of soybean soluble polysaccharides consisting of galacturonan and rhamnogalacturonan, Journal of the Agricultural Chemical Society of Japan, 66, 6, pp. 1301-1313, (2002)
  • [6] 24
  • [7] LIN L H, XIE J H, LIU S C, Et al., Polysaccharide from Mesona chinensis: extraction optimization, physicochemical characterizations and antioxidant activities, International Journal of Biological Macromolecules, 99, pp. 665-673, (2017)
  • [8] WANG S N, SHAO G Q, YANG J J, Et al., Contribution of soybean polysaccharides in digestion of oil-in-water emulsionbased delivery system in an in vitro gastric environment, Food Science and Technology International, 26, 5, pp. 444-452, (2020)
  • [9] (2018)
  • [10] ZHANG X, YU L, BI H T, Et al., Total fraction and characterization of the water-soluble polysaccharides isolated from Panax ginseng C.A.Meyer, Carbohydrate Polymers, 77, pp. 544-522, (2009)
123