Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature

被引:1
|
作者
Huang, Jinwei [1 ,2 ,3 ,4 ]
Wang, Xueying [1 ,2 ,3 ]
Guo, Xiaoyang [1 ,2 ,3 ,5 ]
Liu, Qinghua [1 ,2 ,3 ]
Li, Jun [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, CAS, Qingdao, Peoples R China
[2] Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[5] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
关键词
Sebastes schlegelii; Transient receptor potential (TRP) channels; Temperature; ION-CHANNEL; THERMAL SENSITIVITY; CAPSAICIN-RECEPTOR; CATION CHANNEL; REEF FISHES; HEAT; REPRODUCTION; PERFORMANCE; ACTIVATION; MORTALITY;
D O I
10.1016/j.gene.2024.148317
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Transient Receptor Potential (TRP) channels, essential for sensing environmental stimuli, are widely distributed. Among them, thermosensory TRP channels play a crucial role in temperature sensing and regulation. Sebastes schlegelii, a significant aquatic economic species, exhibits sensitivity to temperature across multiple aspects. In this study, we identified 18 SsTRP proteins using whole-genome scanning. Motif analysis revealed motif 2 in all TRP proteins, with conserved motifs in subfamilies. TRP-related domains, anchored repeats, and ion-transmembrane domains were found. Chromosome analysis showed 18 TRP genes on 11 chromosomes and a scaffold. Phylogenetics classified SsTRPs into four subfamilies: TRPM, TRPA, TRPV, and TRPC. In diverse organisms, four monophyletic subfamilies were identified. Additionally, we identified key TRP genes with significantly upregulated transcription levels under short-term (30 min) and long-term (3 days) exposure at 24 degrees C (optimal elevated temperature) and 27 degrees C (critical high temperature). We propose that genes upregulated at 30 min may be involved in the primary response process of temperature sensing, while genes upregulated at 3 days may participate in the secondary response process of temperature perception. This study lays the foundation for understanding the regulatory mechanisms of TRPs responses to environmental stimuli in S. schlegelii and other fishes.
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页数:12
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