Transient receptor potential (TRP) channels in Sebastes schlegelii: Genome-wide identification and ThermoTRP expression analysis under high-temperature

被引:1
|
作者
Huang, Jinwei [1 ,2 ,3 ,4 ]
Wang, Xueying [1 ,2 ,3 ]
Guo, Xiaoyang [1 ,2 ,3 ,5 ]
Liu, Qinghua [1 ,2 ,3 ]
Li, Jun [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, CAS, Qingdao, Peoples R China
[2] Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[5] Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
关键词
Sebastes schlegelii; Transient receptor potential (TRP) channels; Temperature; ION-CHANNEL; THERMAL SENSITIVITY; CAPSAICIN-RECEPTOR; CATION CHANNEL; REEF FISHES; HEAT; REPRODUCTION; PERFORMANCE; ACTIVATION; MORTALITY;
D O I
10.1016/j.gene.2024.148317
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Transient Receptor Potential (TRP) channels, essential for sensing environmental stimuli, are widely distributed. Among them, thermosensory TRP channels play a crucial role in temperature sensing and regulation. Sebastes schlegelii, a significant aquatic economic species, exhibits sensitivity to temperature across multiple aspects. In this study, we identified 18 SsTRP proteins using whole-genome scanning. Motif analysis revealed motif 2 in all TRP proteins, with conserved motifs in subfamilies. TRP-related domains, anchored repeats, and ion-transmembrane domains were found. Chromosome analysis showed 18 TRP genes on 11 chromosomes and a scaffold. Phylogenetics classified SsTRPs into four subfamilies: TRPM, TRPA, TRPV, and TRPC. In diverse organisms, four monophyletic subfamilies were identified. Additionally, we identified key TRP genes with significantly upregulated transcription levels under short-term (30 min) and long-term (3 days) exposure at 24 degrees C (optimal elevated temperature) and 27 degrees C (critical high temperature). We propose that genes upregulated at 30 min may be involved in the primary response process of temperature sensing, while genes upregulated at 3 days may participate in the secondary response process of temperature perception. This study lays the foundation for understanding the regulatory mechanisms of TRPs responses to environmental stimuli in S. schlegelii and other fishes.
引用
收藏
页数:12
相关论文
共 50 条
  • [41] Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabaci) and expression profiling analysis under temperature stress
    Wang, Xin-Ru
    Wang, Chao
    Ban, Fei-Xue
    Zhu, Dan-Tong
    Liu, Shu-Sheng
    Wang, Xiao-Wei
    INSECT SCIENCE, 2019, 26 (01) : 44 - 57
  • [42] Genome-Wide Identification and Evaluation of New Reference Genes for Gene Expression Analysis Under Temperature and Salinity Stresses in Ciona savignyi
    Huang, Xuena
    Li, Shiguo
    Zhan, Aibin
    FRONTIERS IN GENETICS, 2019, 10
  • [43] Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress
    Sun, Mingyang
    Li, Jingyu
    Xu, Shiqiang
    Gu, Yan
    Wang, Jihua
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (19)
  • [44] Genome-wide identification and expression analysis of the SlNAC gene family in tomato based on a high-quality genome
    Xiuling Chen
    Fulei Mo
    Chunyang Shen
    Lingjun Meng
    Yuxin Zou
    Xiaopeng Xue
    Mozhen Cheng
    Fanyue Meng
    Haonan Qi
    Aoxue Wang
    Horticulture, Environment, and Biotechnology, 2022, 63 : 887 - 901
  • [45] Genome-wide identification and expression analysis of the SlNAC gene family in tomato based on a high-quality genome
    Chen, Xiuling
    Mo, Fulei
    Shen, Chunyang
    Meng, Lingjun
    Zou, Yuxin
    Xue, Xiaopeng
    Cheng, Mozhen
    Meng, Fanyue
    Qi, Haonan
    Wang, Aoxue
    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, 2022, 63 (6) : 887 - 901
  • [46] Genome-wide identification and expression analysis of glutamate receptor-like genes in three Dendrobium species
    Zhu, Miao
    Wang, Xuying
    Li, Xinran
    BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2025, 1869 (06):
  • [47] Genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in Nile tilapia, Oreochromis niloticus
    Cheng, Yun-Ying
    Tao, Wen-Jing
    Chen, Jin-Lin
    Sun, Li-Na
    Zhou, Lin-Yan
    Song, Qiang
    Wang, De-Shou
    GENE, 2015, 569 (01) : 141 - 152
  • [48] The Hsp70 Gene Family in Boleophthalmus pectinirostris: Genome-Wide Identification and Expression Analysis under High Ammonia Stress
    Deng, Zhaochao
    Sun, Shanxiao
    Gao, Tianxiang
    Han, Zhiqiang
    ANIMALS, 2019, 9 (02):
  • [49] Genome-Wide Identification of the LAC Gene Family and Its Expression Analysis Under Stress in Brassica napus
    Ping, Xiaoke
    Wang, Tengyue
    Lin, Na
    Di, Feifei
    Li, Yangyang
    Jian, Hongju
    Wang, Hao
    Lu, Kun
    Li, Jiana
    Xu, Xinfu
    Liu, Liezhao
    MOLECULES, 2019, 24 (10):
  • [50] Genome-wide identification and expression analysis of JmjC domain–containing genes in grape under MTA treatment
    Yi-Zhe Cheng
    Guang-Qi He
    Sheng-Di Yang
    Shuai-Hui Ma
    Jin-Ping Ma
    Fang-Hui-Zi Shang
    Xu-Fei Li
    Hui-Ying Jin
    Da-Long Guo
    Functional & Integrative Genomics, 2022, 22 : 783 - 795