Genome-wide association studies identified OsTMF as a gene regulating rice seed germination under salt stress

被引:3
作者
Liu, Lifeng [1 ,2 ]
Ma, Yanling [1 ]
Zhao, Heng [1 ]
Guo, Lin [1 ]
Guo, Yan [2 ]
Liu, Chun-Ming [1 ,3 ,4 ,5 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China
[2] China Agr Univ, Coll Biol Sci, State Key Lab Plant Environm Resilience, Beijing, Peoples R China
[3] Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
[4] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
[5] Peking Univ, Sch Adv Agr Sci, Beijing, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2024年 / 15卷
基金
国家重点研发计划;
关键词
rice; seed germination; salt stress; GWAS; OsTMF; QUANTITATIVE TRAIT LOCUS; TOLERANCE; PROTEIN; GROWTH; NA+;
D O I
10.3389/fpls.2024.1384246
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Introduction Salt tolerance during seed germination is an important trait for direct seeding and low-cost rice production. Nevertheless, it is still not clear how seed germination under salt stress is regulated genetically. Methods In this study, genome-wide association studies (GWAS) were performed to decipher the genetic basis of seed germination under salt stress using 541 rice varieties collected worldwide. Results and discussion Three quantitative trait loci (QTLs) were identified including qGRG3-1 on chromosome 3, qGRG3-2 on chromosome 5, and qGRG4 on chromosome 4. Assessment of candidate genes in these loci for their responses to salt stress identified a TATA modulatory factor (OsTMF) in qGRG3-2. The expression of OsTMF was up-regulated in both roots and shoots after exposure to salt stress, and OsTMF knockout mutants exhibited delayed seed germination under salt stress. Haplotype analysis showed that rice varieties carrying OsTMF-Hap2 displayed elevated salt tolerance during seed germination. These results provide important knowledge and resources to improve rice seed germination under salt stress in the future.
引用
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页数:11
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