Leucocyte membrane camouflaged poly-lactic-co-glycolic acid (PLGA) nanoparticles containing cannabidiol and paclitaxel against breast cancer therapy

The current investigation focuses on developing multi-drug-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles clothed with leucocyte membrane. Firstly, Paclitaxel (PTX) and Cannabidiol (CBD) were encapsulated in PLGA vesicles which were further coated by a leucocytic membrane (LMc-PTX-CBD-NPs) for efficient targeting of the cancerous cells. Particle size of uncoated and coated nanoparticles was observed to be 149 and 171 nm with the zeta potential of -6 and -10.5 mv, respectively. Later, TEM analysis shows circular particle morphology along with uniform coating of membrane over prepared particles. The prepared formulation possesses prolonged release pattern as well as it shows no hemolytic toxicity. Cytotoxicity analysis in MCF-7 cells of LMc-PTX-CBD-NPs showed better potency than conventional formulation, thus more efficient in targeting cancerous cells. Additionally, greater inhibition of cell migration indicated an improved anti-metastatic effect. Additionally, higher proportion of nuclear fragmentation occurred due to which major changes in cell morphology were observed after treatment with LMc-PTX-CBD-NPs. Thus, prepared formulation could be an efficient approach for successful combination therapy with efficient targeting and reduced side effects to tackle breast cancer.