Evaluation of cytotoxicity, acute toxicity, genotoxicity and antioxidant and antigenotoxicity activities of the sarcotesta fraction of punica granatum L. rich in lectin (PgTel)

被引:1
作者
Coelho, Leila Viviany Araujo [1 ]
de Lima Bento, Elinaldo Francisco [1 ]
Costa, Wendeo Kennedy [1 ]
Ferreira, Magda Rhayanny Assuncao [2 ]
Soares, Luiz Alberto Lira [2 ]
da Silva, Marcia Vanusa [1 ]
Paiva, Patricia Maria Guedes [1 ]
de Oliveira, Alisson Macario [1 ,3 ,4 ]
Napoleao, Thiago Henrique [1 ]
机构
[1] Univ Fed Pernambuco, Ctr Biociencias, Dept Bioquim, Recife, PE, Brazil
[2] Univ Fed Pernambuco, Ctr Ciencias Saude, Dept Farm, Recife, PE, Brazil
[3] Univ Estadual Paraiba, Programa Pos Grad Ciencias Farmaceut, Campina Grande, PB, Brazil
[4] Univ Fed Pernambuco, Dept Bioquim, BR-50670901 Recife, PE, Brazil
来源
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES | 2024年 / 87卷 / 14期
关键词
Pomegranate; toxicity assessment; acute toxicity; swiss female mice; antioxidant; OXIDATIVE STRESS; POMEGRANATE PEEL; EXTRACT; QUANTITATION; MANAGEMENT;
D O I
10.1080/15287394.2024.2351476
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Punica granatum, popularly known as pomegranate, is a fruit tree with wide worldwide distribution, containing numerous phytochemicals of great medicinal value. The aim of the present study was to determine the phytochemical profile and antioxidant potential of a protein fraction (PF) derived from P. granatum sarcotesta which is rich in lectin. In addition, the acute oral toxicity, genotoxicity and antigenotoxicity of this protein fraction (PF) from P. granatum sarcotesta was measured. The phytochemical profile of PF was determined using HPLC. The in vitro antioxidant effect was assessed using the methods of total antioxidant capacity (TAC) and DPPH and ABTS+ radical scavenging. Acute oral toxicity was determined in female Swiss mice administered a single dose of 2000 mg/kg. This PF was examined for genotoxicity and antigenotoxicity at doses of 500, 1000 and 2000 mg/kg, utilizing mouse peripheral blood cells. Phytochemical characterization detected a high content of ellagic acid and antioxidant capacity similar to that of ascorbic acid (positive control). PF was not toxic (LD50 >2000 mg/kg) and did not exert a genotoxic effect in mice. PF protected the DNA of peripheral blood cells against damage induced by cyclophosphamide. In conclusion, this PF fraction exhibited significant antioxidant activity without initiating toxic or genotoxic responses in mice.
引用
收藏
页码:592 / 603
页数:12
相关论文
共 58 条
  • [1] Comparative Study on the Influence of Some Medicinal Plants on Diabetes Induced by Streptozotocin in Male Rats
    Almalki, Daklallah A.
    Alghamdi, Sameera A.
    Al-Attar, Atef M.
    [J]. BIOMED RESEARCH INTERNATIONAL, 2019, 2019
  • [2] Safety profile and gender specific differences of a methanol extract of Eriosema laurentii (Leguminosae) in acute and subchronic (28 days) oral toxicity studies in Wistar rats
    Ateba, Sylvin Benjamin
    Simo, Rudy Valdes
    Mbanya, Jean Claude
    Krenn, Liselotte
    Njamen, Dieudonne
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 2014, 65 : 27 - 32
  • [3] Is it safe to consume traditional medicinal plants during pregnancy?
    Bernstein, Nirit
    Akram, Muhammad
    Yaniv-Bachrach, Zohara
    Daniyal, Muhammad
    [J]. PHYTOTHERAPY RESEARCH, 2021, 35 (04) : 1908 - 1924
  • [4] BING DH, 1967, P SOC EXP BIOL MED, V124, P1166
  • [5] Applications of Essential Oils and Plant Extracts in Different Industries
    Bolouri, Parisa
    Salami, Robab
    Kouhi, Shaghayegh
    Kordi, Masoumeh
    Asgari Lajayer, Behnam
    Hadian, Javad
    Astatkie, Tess
    [J]. MOLECULES, 2022, 27 (24):
  • [6] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [7] BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25
  • [8] Cavdar HK, 2017, FOOD TECHNOL BIOTECH, V55, P86, DOI [10.17113/ft b.55.01.17.4638, 10.17113/ftb.55.01.17.4638]
  • [9] Chen Z, 2020, FREE RADICAL BIO MED, V152, P116, DOI 10.1016/j.freeradbiomed.2020.02.025
  • [10] Cytotoxicity, oral toxicity, genotoxicity, and mutagenicity evaluation of essential oil from Psidium glaziovianum Kiaersk leaves
    Costa, Wendeo Kennedy
    do Nascimento, Matheus Ferreira
    Barbosa, Edipo Lucas Soares
    Souza, Talita Giselly dos Santos
    Chagas, Cristiano Aparecido
    Napolea, Thiago Henrique
    Correia, Maria Tereza dos Santos
    Brayner, Fabio Andre
    de Oliveira, Alisson Macario
    da Silva, Marcia Vanusa
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2023, 303