Ameliorating effects of Lactobacillus probiotics on cadmium-induced hepatotoxicity, inflammation, and oxidative stress in Wistar rats

被引：0
作者：
Kohan A. [1 ]
Keshtmand Z. [1 ]
机构：
[1] Departmant of Biology, Central Tehran Branch, Islamic Azad University, Tehran
来源：
Comparative Clinical Pathology | 2024年 / 33卷 / 4期
关键词：
Anti-inflammatory agents; Antioxidants; Cadmium chloride; Liver; Probiotic;
D O I：
10.1007/s00580-024-03583-5
中图分类号：
学科分类号：
摘要：
Cadmium (Cd) causes significant oxidative stress, which can lead to major clinical complications and tissue destruction. In this study, the antioxidant and anti-inflammatory effects of a mixture of Iranian probiotic strains were evaluated in Cd-exposed rats. Twenty-one adult male Wistar rats were randomized into three groups of seven rats: the control group, Cd-treated (3 mg/kg, a single dose) animals, and the rats treated with Cd + probiotic (Cd + Pro) for 30 days. Histological changes were assessed using hematoxylin and eosin staining, as well as Masson’s trachoma staining. The qRT-PCR method was used for evaluating pro-inflammatory genes’ expression. Liver biomarkers (ALT, ALP, and AST), biochemical enzymes, and blood parameters were measured using commercial kits, and hematological parameters were determined in both Cd- and the Cd + Pro–treated groups. Histopathologically, results revealed that the native probiotic mixture improved the function of Cd-treated liver cells, compared with Cd-treated rats. Also, positive changes of blood parameters and liver biomarker were observed in the treated group compared with the Cd-treated group. There was a down-regulation of TNF-α and IL-6 genes’ expression in Cd + Pro–treated compared with the Cd-treated group. In addition, Cd + Pro–treated rats displayed lower production of lipid peroxides, lower levels of hepatic toxicity biomarkers, and increased levels of CAT and SOD antioxidant biomarkers compared to Cd-treated animals. Iranian native probiotics showed antioxidant and anti-inflammatory properties, and it is suggested to investigate their health benefits in animal studies in the future. © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2024.
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页码：653 / 664
页数：11
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