The sampling strategy of oral microbiome

There are multiple habitats in the oral cavity with bacteria, fungi, viruses, and protozoa residing in, which together constitute the oral micro-ecosystem. These microflorae in the oral cavity primarily include saliva, supragingival dental plaque, subgingival dental plaque, submucosal plaque around implants, plaque in root canals, and plaque on the mucosal surface. The interest and knowledge of the microbiome have dynamically increased with the advancement of technology. Therefore, a reliable, feasible, and practical sampling strategy for the oral microbiome is required for the investigation. This paper introduced the sampling strategy of oral microorganisms, consisting of sample collection, transport, processing, and storage. The materials and devices involved in this study are all commonly used in clinical practice or laboratory. The feasibility and reliability of the sampling methods described in this paper have been verified by multiple studies. Oral microbiome attracts substantial attention, and a reliable, feasible, and practical sampling strategy for the oral microbiome is required. The habitats of microorganisms in the oral cavity primarily include saliva, supragingival area, subgingival/submucosal area, infected root canals, and mucosal surface. The sampling strategy of oral microbiota is described in this study, which consists of sample collection, transport, processing, and storage. The sampling sites in the oral cavity primarily include saliva, supragingival area, subgingival/submucosal area, infected root canals, and mucosal surface. image