RUNX1-induced upregulation of PTGS2 enhances cell growth, migration and invasion in colorectal cancer cells

被引：8

作者：

Zheng, Weiwei ^{[1
,2
,3
]}

Guo, Yingchang ^{[4
]}

Kahar, Aihemaiti ^{[2
]}

Bai, Junwei ^{[1
]}

Zhu, Qinhui ^{[5
]}

Huang, Xinli ^{[6
]}

Li, Yuan ^{[7
]}

Xu, Bingyi ^{[8
]}

Jia, Xueshan ^{[9
]}

Wu, Gang ^{[1
]}

Zhang, Chao ^{[1
]}

Zhu, Yuanzeng ^{[1
]}

机构：

[1] Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Gastrointestinal Surg, Peoples Hosp, 7 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China

[2] Red Star Hosp 13th Div Xinjiang Prod & Construct C, Hepatobiliary Gastrointestinal Surg Dept, Hami 839000, Xinjiang, Peoples R China

[3] Xinxiang Med Coll, Affiliated Hosp, Xinxiang 453000, Henan, Peoples R China

[4] Xinxiang Med Coll, Dept Intervent Therapy, Affiliated Hosp 1, Xinxiang 453000, Henan, Peoples R China

[5] Shangcai Peoples Hosp, Dept Gen Surg, Zhumadian 463800, Henan Province, Peoples R China

[6] Suiping Peoples Hosp, Dept Gen Surg, Zhumadian 463100, Henan, Peoples R China

[7] Zhengzhou Univ, Peoples Hosp, Henan Prov Peoples Hosp, Dept Anesthesiol, Zhengzhou 450003, Henan, Peoples R China

[8] Weihui Peoples Hosp, Weihui 453100, Henan, Peoples R China

[9] Zhengzhou Univ, Henan Prov Peoples Hosp, Dev Dept, Peoples Hosp, Zhengzhou 450003, Henan, Peoples R China

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Colorectal cancer; PTGS2; Transcription factor (TF); RUNX1; CYCLOOXYGENASE-2; EXPRESSION; OVER-EXPRESSION; COX-2; RUNX1; GENE; PROLIFERATION; ASSOCIATION; BIOMARKERS; BREAST;

D O I：

10.1038/s41598-024-60296-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Colorectal cancer (CRC) arises via the progressive accumulation of dysregulation in key genes including oncogenes and tumor-suppressor genes. Prostaglandin-endoperoxide synthase 2 (PTGS2, also called COX2) acts as an oncogenic driver in CRC. Here, we explored the upstream transcription factors (TFs) responsible for elevating PTGS2 expression in CRC cells. The results showed that PTGS2 silencing repressed cell growth, migration and invasion in HCT116 and SW480 CRC cells. The two fragments (499-981 bp) and (1053-1434 bp) were confirmed as the core TF binding profiles of the PTGS2 promoter. PTGS2 expression positively correlated with RUNX1 level in colon adenocarcinoma (COAD) samples using the TCGA-COAD dataset. Furthermore, RUNX1 acted as a positive regulator of PTGS2 expression by promoting transcriptional activation of the PTGS2 promoter via the 1086-1096 bp binding motif. In conclusion, our study demonstrates that PTGS2 upregulation induced by the TF RUNX1 promotes CRC cell growth, migration and invasion, providing an increased rationale for the use of PTGS2 inhibitors in CRC prevention and treatment.

引用

页数：13

共 54 条

[1] Prostaglandin-Endoperoxide Synthase 2 (PTGS2) in the Oviduct: Roles in Fertilization and Early Embryo Development [J].