Reduced ossification caused by 3D simulated microgravity exposure is short-term in larval zebrafish

被引:0
作者
Carvajal-Agudelo, Juan D. [1 ]
Eaton, Jordan [1 ,2 ]
Franz-Odendaal, Tamara A. [1 ]
机构
[1] Mt St Vincent Univ, Dept Biol, Halifax, NS B3M 2J6, Canada
[2] St Marys Univ, Dept Biol, Halifax, NS, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Random positioning machine; Operculum; Tnfsf11; Rankl; Collagen x; Ossification; GENE-EXPRESSION; MODELED MICROGRAVITY; BONE; OSTEOBLAST; OSTEOCLASTOGENESIS; HYPERGRAVITY; OSTEOPOROSIS; VIBRATION; IMPACT; DIFFERENTIATION;
D O I
10.1016/j.lssr.2024.02.006
中图分类号
P1 [天文学];
学科分类号
0704 ;
摘要
Understanding how skeletal tissues respond to microgravity is ever more important with the increased interest in human space travel. Here, we exposed larval Danio rerio at 3.5 dpf to simulated microgravity (SMG) using a 3D mode of rotation in a ground-based experiment and then studied different cellular, molecular, and morphological bone responses both immediately after exposure and one week later. Our results indicate an overall decrease in ossification in several developing skeletal elements immediately after SMG exposure with the exception of the otoliths, however ossification returns to normal levels seven days after exposure. Coincident with the reduction in overall ossification tnfsf11 (RANKL) expression is highly elevated after 24 h of SMG exposure and also returns to normal levels seven days after exposure. We also show that genes associated with osteoblasts are unaffected immediately after SMG exposure. Thus, the observed reduction in ossification is primarily the result of a high level of bone resorption. This study sheds insight into the nuances of how osteoblasts and osteoclasts in the skeleton of a vertebrate organism respond to an external environmental disturbance, in this case simulated microgravity.
引用
收藏
页码:127 / 135
页数:9
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