Distribution of Typical Resistant Bacteria and Resistance Genes in Source Water of the Middle and Lower Reaches of the Yellow River

被引：0

作者：

Min W. ^{[1
]}

Gao M.-C. ^{[2
]}

Sun S.-F. ^{[1
,3
]}

Song Q.-Q. ^{[1
]}

Qiu L.-P. ^{[3
,4
]}

机构：

[1] School of Civil Engineering and Architecture, University of Jinan, Jinan

[2] School of Conservancy and Environment, University of Jinan, Jinan

[3] Shandong Province Engineering Technology Research Center for Water Purification Functional Material, Jinan

[4] School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan

来源：

Huanjing Kexue/Environmental Science | 2024年 / 45卷 / 02期

关键词：

antibiotic resistance genes（ARGs）; antibiotic resistant bacteria（ARB）; quantitative-polymerase chain reaction （qPCR）; spatial and temporal distribution; Yellow River Basin;

D O I：

10.13227/j.hjkx.202211314

中图分类号：

学科分类号：

摘要：

The Yellow River water of an urban area located in the middle and lower reaches of the Yellow River was taken as the research object，in which the seasonal and along-range distribution of total culturable bacteria，typical antibiotic resistant bacteria（amoxicillin resistant bacteria and sulfamethoxazole-resistant bacteria），and their corresponding typical resistance genes［β-lactam resistance gene（blaCTX-M）and sulfamamide resistance genes（sulI and sul ），as well as int 1 were investigated. The results showed that the total culturable bacteria，β-lactam-resistant bacteria and sulfonamide-resistant bacteria in the Yellow River Basin were significantly affected by temperature and human activities. The composition and quantity of their genera had obvious spatiotemporal distribution characteristics，in which Bacillus and Pseudomonas were dominant in the composition and number of bacteria. The abundance of resistance genes decreased with the decrease in temperature. The proportion of β-lactam resistance genes in the total genes was higher than that of sulfanilamide genes，and sulI was the dominant gene in sulfanilamide genes. Correlation analysis showed that class Ⅰ integron played an important role in accelerating the spread of resistance genes. This study offers insight into the status quo of water resistance pollution in the Yellow River and provides theoretical support for the risk assessment of resistance genes in the middle and lower reaches of the Yellow River Basin. © 2024 Science Press. All rights reserved.

引用

页码：837 / 843

页数：6

共 48 条

[1]

Nguyen A Q，, Vu H P，, Nguyen L N，, Et al., Monitoring antibiotic resistance genes in wastewater treatment：Current strategies and future challenges［J］, Science of the Total Environment, (2021)

[2]

Zheng D S，, Yin G Y，, Liu M，, Et al., A systematic review of antibiotics and antibiotic resistance genes in estuarine and coastal environments［J］, Science of the Total Environment, (2021)

[3]

Pruden A，, Pei R T，, Storteboom H，, Et al., Antibiotic resistance genes as emerging contaminants： studies in northern colorado［J］, Environmental Science & Technology, 40, 23, pp. 7445-7450, (2006)

[4]

Li B，, Qiu Y，, Song Y Q，, Et al., Dissecting horizontal and vertical gene transfer of antibiotic resistance plasmid in bacterial community using microfluidics［J］, Environment International, 131, (2019)

[5]

Zhang Y P，, Niu Z G，, Zhang Y，, Et al., Occurrence of intracellular and extracellular antibiotic resistance genes in coastal areas of Bohai Bay （China） and the factors affecting them ［J］, Environmental Pollution, 236, pp. 126-136, (2018)

[6]

Gillings M R., Lateral gene transfer，bacterial genome evolution，and the Anthropocene［J］, Annals of the New York Academy of Sciences, 1389, 1, pp. 20-36, (2017)

[7]

Han Z M, Et al., Antibiotic resistomes in drinking water sources across a large geographical scale：Multiple drivers and co-occurrence with opportunistic bacterial pathogens ［J］, Water Research, (2020)

[8]

Yang Y，, Zhou R J，, Chen B W，, Et al., Characterization of airborne antibiotic resistance genes from typical bioaerosol emission sources in the urban environment using metagenomic approach ［J］, Chemosphere, 213, pp. 463-471, (2018)

[9]

Liu X H，, Lu S Y，, Guo W，, Et al., Antibiotics in the aquatic environments：A review of lakes，China［J］, Science of the total Environment, 627, pp. 1195-1208, (2018)

[10]

Qin R, Yu Q G, Liu Z Y，, Et al., Co-occurrence of tetracycline antibiotic resistance genes and microbial communities in plateau wetlands under the influence of human activities ［J］, Environmental Science, 44, 1, pp. 169-179, (2023)

← 1 2 3 4 5 →