Restoring Treg/Th17 cell balance in ulcerative colitis through HRas silencing and MAPK pathway inhibition

被引：2
作者：
Wang, Siwei [1 ]
Su, Wenhao [2 ]
Wu, Xiaohan [2 ]
Dong, Weiguo [2 ]
机构：
[1] Capital Med Univ, Beijing Friendship Hosp, Dept Pathol, Beijing 100050, Peoples R China
[2] Wuhan Univ, Renmin Hosp, Dept Gastroenterol, 238 Jiefang Rd,99 Zhang Zhidong Rd, Wuhan 430060, Hubei, Peoples R China
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2024年 / 130卷
基金：
美国国家科学基金会;
关键词：
Ulcerative colitis; HRas silencing; MAPK pathway; Treg cells; Th17; cells; Treg/Th17; imbalance; RNA sequencing; SIGNALING PATHWAY; PATHOGENESIS; IMMUNITY;
D O I：
10.1016/j.intimp.2024.111608
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This study investigates HRas-dependent mechanisms in the disruption of regulatory T (Treg) cells and T helper 17 (Th17) cells balance in ulcerative colitis (UC). Comprehensive RNA sequencing and bioinformatics analyses revealed elevated HRas and MAPK pathway -related protein expression in UC samples. Using a murine UC model induced by dextran sulfate sodium (DSS), HRas silencing was found to promote Treg cell differentiation and suppress Th17 cell production, effectively restoring balance. Inactivation of the MAPK pathway played a pivotal role in this rebalancing effect. In vivo experiments further confirmed that HRas silencing mitigated colon tissue damage in DSS-induced mice, emphasizing its potential as a therapeutic strategy for UC.
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页数：13
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