Therapeutic effect of human umbilical cord mesenchymal stem cells and their conditioned medium on LPS-induced endometritis in mice

被引：2
作者：
Li, Jingyi [1 ]
Yin, Xiaodi [1 ]
Du, Ming [1 ]
Wang, Caiyi [2 ]
Zou, Feng [1 ]
Ma, Jun [1 ]
Song, Yuxia [2 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 2, Dept Lab Med, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 2, Dept Reprod Med, Zhengzhou, Peoples R China
来源：
TISSUE & CELL | 2024年 / 88卷
关键词：
Human umbilical cord mesenchymal stem cells; hUC-MSCs; Mesenchymal stem cell conditioned medium; MSC-CM; Acute inflammatory model of endometritis in; mice; Lipopolysaccharide; LPS; INFLAMMATORY RESPONSE; TLR4/NF-KAPPA-B; PATHWAY;
D O I：
10.1016/j.tice.2024.102346
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Aim: To explore the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) and their conditioned medium (MSC-CM) in repairing the endometritis mouse model in vivo. Methods: Lipopolysaccharide (LPS) was used to induce acute inflammation in endometritis mouse model. Mice were treated in six groups: control group (PBS), model group (LPS), LPS+MSC-CM (6 h) group, LPS+MSC-CM (12 h) group, LPS+MSCs (6 h) group and LPS+MSCs (12 h) group. Morphological and histological changes of mouse uterus were observed, and mouse uterine inflammation index myeloperoxidase (MPO) and related immune index TNF-alpha, IL-6 and IL-1 beta levels were detected by ELISA. Results: There exist remarkable inflammatory response and an obvious increase in the value of MPO, TNF-alpha, IL-1 beta and IL-6 in the endometritis mouse model compared with the control group. Morphological and histological appearances were relieved after treated with hUC-MSCs and MSC-CM. Besides, the value of MPO, TNF-alpha, IL-1 beta and IL-6 showed different degrees of decline. In comparison with LPS+MSC-CM (12 h) and LPS+MSCs (12 h) group, there was significant decrease in inflammatory indicators in LPS+MSC-CM (6 h) and LPS+MSCs (6 h) group. Conclusions: Intrauterine infusion of hUC-MSCs and MSC-CM can alleviate LPS induced endometritis.
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页数：8
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