Therapeutic effect of human umbilical cord mesenchymal stem cells and their conditioned medium on LPS-induced endometritis in mice

被引：2
作者：
Li, Jingyi [1 ]
Yin, Xiaodi [1 ]
Du, Ming [1 ]
Wang, Caiyi [2 ]
Zou, Feng [1 ]
Ma, Jun [1 ]
Song, Yuxia [2 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 2, Dept Lab Med, Zhengzhou, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 2, Dept Reprod Med, Zhengzhou, Peoples R China
来源：
TISSUE & CELL | 2024年 / 88卷
关键词：
Human umbilical cord mesenchymal stem cells; hUC-MSCs; Mesenchymal stem cell conditioned medium; MSC-CM; Acute inflammatory model of endometritis in; mice; Lipopolysaccharide; LPS; INFLAMMATORY RESPONSE; TLR4/NF-KAPPA-B; PATHWAY;
D O I：
10.1016/j.tice.2024.102346
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Aim: To explore the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) and their conditioned medium (MSC-CM) in repairing the endometritis mouse model in vivo. Methods: Lipopolysaccharide (LPS) was used to induce acute inflammation in endometritis mouse model. Mice were treated in six groups: control group (PBS), model group (LPS), LPS+MSC-CM (6 h) group, LPS+MSC-CM (12 h) group, LPS+MSCs (6 h) group and LPS+MSCs (12 h) group. Morphological and histological changes of mouse uterus were observed, and mouse uterine inflammation index myeloperoxidase (MPO) and related immune index TNF-alpha, IL-6 and IL-1 beta levels were detected by ELISA. Results: There exist remarkable inflammatory response and an obvious increase in the value of MPO, TNF-alpha, IL-1 beta and IL-6 in the endometritis mouse model compared with the control group. Morphological and histological appearances were relieved after treated with hUC-MSCs and MSC-CM. Besides, the value of MPO, TNF-alpha, IL-1 beta and IL-6 showed different degrees of decline. In comparison with LPS+MSC-CM (12 h) and LPS+MSCs (12 h) group, there was significant decrease in inflammatory indicators in LPS+MSC-CM (6 h) and LPS+MSCs (6 h) group. Conclusions: Intrauterine infusion of hUC-MSCs and MSC-CM can alleviate LPS induced endometritis.
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页数：8
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共 38 条
[1]   Neuroprotective Effects of Conditioned Medium of Mesenchymal Stem Cells (MSC-CM) as a Therapy for Ischemic Stroke Recovery: A Systematic Review [J].
Behzadifard, Mahin ;
Aboutaleb, Nahid ;
Dolatshahi, Mojtaba ;
Khorramizadeh, Maryam ;
Jahangiri, Hamzeh Mirshekari ;
Kord, Zeynab ;
Nazarinia, Donya .
NEUROCHEMICAL RESEARCH, 2023, 48 (05) :1280-1292
[2]   Very small embryonic-like stem cells (VSELs) regenerate whereas mesenchymal stromal cells (MSCs) rejuvenate diseased reproductive tissues [J].
Bhartiya, Deepa ;
Singh, Pushpa ;
Sharma, Diksha ;
Kaushik, Ankita .
STEM CELL REVIEWS AND REPORTS, 2022, 18 (05) :1718-1727
[3]   Interaction Between Chronic Endometritis Caused Endometrial Microbiota Disorder and Endometrial Immune Environment Change in Recurrent Implantation Failure [J].
Chen, Peigen ;
Chen, Panyu ;
Guo, Yingchun ;
Fang, Cong ;
Li, Tingting .
FRONTIERS IN IMMUNOLOGY, 2021, 12
[4]   lncRNA NEAT1 ameliorates LPS-induced inflammation in MG63 cells by activating autophagy and suppressing the NLRP3 inflammasome [J].
Dai, Wenyu ;
Wang, Manyi ;
Wang, Peiqi ;
Wen, Ji ;
Wang, Jiangyue ;
Cha, Sa ;
Xiao, Xueling ;
He, Yiruo ;
Shu, Rui ;
Bai, Ding .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2021, 47 (02) :607-620
[5]   Chronic endometritis and embryo implantation: the great illusion [J].
de Ziegler, Dominique .
FERTILITY AND STERILITY, 2022, 118 (04) :637-638
[6]   Mesenchymal stromal cell-associated migrasomes: a new source of chemoattractant for cells of hematopoietic origin [J].
Deniz, Ilker A. ;
Karbanova, Jana ;
Wobus, Manja ;
Bornhaeuser, Martin ;
Wimberger, Pauline ;
Kuhlmann, Jan Dominik ;
Corbeil, Denis .
CELL COMMUNICATION AND SIGNALING, 2023, 21 (01)
[7]   Human Umbilical Cord Mesenchymal Stem Cells: A New Era for Stem Cell Therapy [J].
Ding, Dah-Ching ;
Chang, Yu-Hsun ;
Shyu, Woei-Cherng ;
Lin, Shinn-Zong .
CELL TRANSPLANTATION, 2015, 24 (03) :339-347
[8]   Stem cell-based therapy for human diseases [J].
Hoang, Duc M. ;
Pham, Phuong T. ;
Bach, Trung Q. ;
Ngo, Anh T. L. ;
Nguyen, Quyen T. ;
Phan, Trang T. K. ;
Nguyen, Giang H. ;
Le, Phuong T. T. ;
Hoang, Van T. ;
Forsyth, Nicholas R. ;
Heke, Michael ;
Liem Thanh Nguyen .
SIGNAL TRANSDUCTION AND TARGETED THERAPY, 2022, 7 (01)
[9]   Clostridium tyrobutyricum alleviates Staphylococcus aureus-induced endometritis in mice by inhibiting endometrial barrier disruption and inflammatory response [J].
Hu, Xiaoyu ;
Guo, Jian ;
Xu, Mingyue ;
Jiang, Peng ;
Yuan, Xin ;
Zhao, Caijun ;
Maimai, T. ;
Cao, Yongguo ;
Zhang, Naisheng ;
Fu, Yunhe .
FOOD & FUNCTION, 2019, 10 (10) :6699-6710
[10]   Soy isoflavones improves endometrial barrier through tight junction gene expression [J].
Kiatprasert, Pongpat ;
Deachapunya, Chatsri ;
Benjanirat, Chutamas ;
Poonyachoti, Sutthasinee .
REPRODUCTION, 2015, 149 (03) :269-280
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