Antioxidant Activity of Total Flavonoids Extracts from Folium of Artemisiae argyi in Vitro and in Vivo

被引：0
|
作者：
Hu Q. [1 ]
Li J. [1 ]
Liu D. [1 ]
Cao Y. [1 ]
机构：
[1] Key Laboratory of Ministry of Education on Traditional Chinese Medicine Resource and Compound Prescription, Pharmacy Faculty, Hubei University of Chinese Medicine, Wuhan
来源：
Science and Technology of Food Industry | 2021年 / 42卷 / 06期
关键词：
antioxidase; antioxidation activity; Caenorhabditis elegans; flavonoids from folium of Artemisia argyi; main components; oxidative stress;
D O I：
10.13386/j.issn1002-0306.2020070227
中图分类号：
学科分类号：
摘要：
To explore the content of the main components of the total flavonoids extract from folium of Artemisia argyi(TFEFAA) and its antioxidant activity in vivo and in vitro. In this paper, high-performance liquid chromatography(HPLC) was used to simultaneously determine the content of eupatilin and jaceosidin in TFEFAA, the antioxidant activity of TFEFAA in vitro was investigated with the scavenging rate of 1, 1-diphenyl-2-trinitrophenylhydrazine(DPPH), 2, 2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt(ABTS) and hydroxyl radical and total reducing power as indexes. Caenorhabditis elegans(C. elegans) were cultured in nematode growth media containing 0, 50 and 100 μg/mL TFEFAA respectively. The effects of TFEFAA on heat stress and H2O2 oxidative stress of C. elegans were studied. At the same time, the related antioxidant enzyme activity and malondialdehyde(MDA) content in C. elegans were detected. The results showed that the contents of eupatilin and jaceosidin were 0.2% and 0.06% respectively and TFEFAA had a significant scavenging effect on DPPH radical, ABTS+ radical and hydroxyl radical, and its IC50 were 96.51, 67.73 and 279.38 μg/mL, respectively. The scavenging ability had a dose-effect relationship with TFEFAA, and it also had a strong total reducing power. 100 μg/mL TFEFAA can significantly prolong the survival time of nematodes under heat stress and H2O2 oxidative stress(P<0.01), at the same time significantly increase the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in nematodes(P<0.01), and significantly reduce the MDA contents in nematodes(P<0.05). TFEFAA had good antioxidant activity in vivo and in vitro, and its mechanism may be related to improving antioxidant enzyme activity in vivo. Therefore, TFEFAA would be expected to be developed into a new natural green food antioxidant. © 2021 Science and Technology of Food Industry. All rights reserved.
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页码：304 / 309
页数：5
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  • [1] Kandola K, Bowman A, Birch-Machin M A., Oxidative stress-a key emerging impact factor in health,ageing,lifestyle and aesthetics[J], International Journal of Cosmetic Science, 37, pp. 1-8, (2015)
  • [2] Ruan D, Wang W C, Lin C X, Et al., Effects of curcumin on performance,antioxidation,intestinal barrier and mitochondrial function in ducks fed corn contaminated with ochratoxin A[J], Animal, 13, 1, pp. 42-52, (2019)
  • [3] Deng Y, Lei T W, Li H M, Et al., ERK5/KLF<sub>2</sub> activation is involved in the reducing effects of puerarin on monocyte adhesion to endothelial cells and atherosclerotic lesion in apolipoprotein E-deficient mice[J], Biochimica et Biophysica Acta(BBA)-Molecular Basis of Disease, 1864, 8, pp. 2590-2599, (2018)
  • [4] Yun C Y, Jung Y, Chun W, Et al., Anti-inflammatory effects of Artemisia leaf extract in mice with contact dermatitis in vitro and in vivo[J], Mediators of Inflammation, 2016, (2016)
  • [5] Li Y Y, Wu H, Dong Y G, Et al., Application of eupatilin in the treatment of osteosarcoma[J], Oncology Letters, 10, 4, pp. 2505-2510, (2015)
  • [6] Wang Q Q, Yang F, Guo W, Et al., Caenorhabditis elegans in Chinese medicinal studies:making the case for aging and neurodegeneration[J], Rejuvenation Research, 17, 2, pp. 205-208, (2014)
  • [7] Jin S Y, Li D Q, Lu S, Et al., Ethanol extracts of Panax notoginseng increase lifespan and protect against oxidative stress in Caenorhabditis elegans via the insulin/IGF-1 signaling pathway[J], Journal of Functional Foods, 58, pp. 218-226, (2019)
  • [8] Xiao S, Zhang Y Q, Xie J B, Et al., Ultrasonic-assisted extraction of squalene and vitamin E based oil from Zizyphi Spinosae Semen and evaluation of its antioxidant activity[J], Journal of Food Measurement and Characterization, 12, 4, pp. 2844-2854, (2018)
  • [9] Chen W, Lin H R, Wei C M, Et al., Echinacoside,a phenylethanoid glycoside from Cistanche deserticola,extends lifespan of Caenorhabditis elegans and protects from A β-induced toxicity[J], Biogerontology, 19, 1, pp. 47-65, (2018)
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