Current Knowledge of Base Editing and Prime Editing

被引：4

作者：

Averina, O. A. ^{[1
,2
,3
]}

Kuznetsova, S. A. ^{[1
]}

Permyakov, O. A. ^{[1
,3
]}

Sergiev, P. V. ^{[1
,2
,3
]}

机构：

[1] Moscow State Univ, Inst Funct Genom, Moscow 119991, Russia

[2] Moscow State Univ, Belozersky Inst Physico Chem Biol, Moscow 119991, Russia

[3] Moscow State Univ, Chem Fac, Moscow 119991, Russia

来源：

MOLECULAR BIOLOGY | 2024年 / 58卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

CRISPR/Cas technology; base editing; nucleoside deaminases; prime editing; reverse transcriptase; repair systems; GENOMIC DNA; OFF-TARGET; CRISPR-CAS9; EDITORS;

D O I：

10.1134/S0026893324700195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modern genetic engineering technologies, such as base editing and prime editing (PE), have proven to provide the efficient and reliable genome editing tools that obviate the need for donor templates and double-strand breaks (DSBs) introduced in DNA. Relatively new, they quickly gained recognition for their accuracy, simplicity, and multiplexing capabilities. The review summarizes the new literature on the technologies and considers their architecture, methods to create editors, specificity, efficiency, and versatility. Advantages and disadvantages of the editors are discussed along with their prospective use in basic and applied research. The review may be useful for planning genome editing studies and analyzing their results to solve various problems of fundamental biology, biotechnology, medicine, and agriculture.

引用

页码：571 / 587

页数：17

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