Network pharmacology-based research into the mechanism of ferulic acid on acute lung injury through enhancing transepithelial sodium transport

Ethnopharmacological relevance: Ferulic acid (FA) has shown potential therapeutic applications in treating lung diseases. However, the underlying mechanisms by which FA ameliorates acute lung injury (ALI) have not been distinctly elucidated. Aim of the study: The project aims to observe the therapeutic effects of FA on lipopolysaccharide-induced ALI and to elucidate its specific mechanisms in regulating epithelial sodium channel (ENaC), which majors in alveolar fluid clearance during ALI. Materials and methods: In this study, the possible pathways of FA were determined through network pharmacology analyses. The mechanisms of FA in ALI were verified by in vivo mouse model and in vitro studies, including primary alveolar epithelial type 2 cells and three-dimensional alveolar organoid models. Results: FA ameliorated ALI by improving lung pathological changes, reducing pulmonary edema, and upregulating the alpha/gamma-ENaC expression in C57BL/J male mice. Simultaneously, FA was observed to augment ENaC levels in both three-dimensional alveolar organoid and alveolar epithelial type 2 cells models. Network pharmacology techniques and experimental data from inhibition or knockdown of IkappaB kinase beta (IKK beta) proved that FA reduced the phosphorylation of IKK beta/nuclear factor-kappaB (NF-kappa B) and eliminated the lipopolysaccharideinhibited expression of ENaC, which could be regulated by nuclear protein NF-kappa B p65 directly. Conclusions: FA could enhance the expression of ENaC at least in part by inhibiting the IKK beta/NF-kappa B signaling pathway, which may potentially pave the way for promising treatment of ALI.