Long-term effect of acetate and biochar addition on enrichment and activity of denitrifying anaerobic methane oxidation microbes

被引：4
作者：
Ding J. [1 ,2 ]
Qin F. [2 ]
Li C. [2 ]
Tang M. [2 ]
机构：
[1] National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Jiangsu, Suzhou
[2] School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou
来源：
Chemosphere | 2023年 / 338卷
基金：
中国国家自然科学基金;
关键词：
Acetate; Biochar; DAMO bacteria; Denitrification anaerobic methane oxidation (DAMO); Electron transfer;
D O I：
10.1016/j.chemosphere.2023.139642
中图分类号：
学科分类号：
摘要：
The denitrifying anaerobic methane oxidation (DAMO) process plays a crucial role in the global carbon/nitrogen cycles and methane emission control, and also has application potential in biological wastewater treatment. However, given that DAMO microbes are susceptible to external conditions such as additional carbon source in the system, it is essential to evaluate the effect of alternative carbon substance on the enrichment efficiency and metabolic activity of DAMO microbes. To this end, this study investigated the effect of acetate (0.1 mmol/L-R2, 0.5 mmol/L-R3) and biochar addition (R4) on the enrichment and activity of DAMO microbes. The long-term operation showed that the NO2−-N and CH4 consumption rates in the reactors almost presented the sequence of R4>R2>R3>R1. However, the short-term activity test with isotope labelling showed the sequence of R2>R4>R1>R3. Furthermore, the addition of acetate and biochar improved the electrochemical activity and extracellular polymeric substance (EPS) secretion in the systems. In R4 reactor, the proportion of DAMO bacteria was the highest (7.20%), indicating that the addition of biochar could promote the enrichment of DAMO bacteria, and Thauera was co-enriched with the proportion increasing from 0.26% to 6.73%. While in R1, R2 and R3 reactors, DAMO bacteria were enriched with relatively low abundances (0.10%, 0.23%, 0.15%, respectively), together with methanogens and denitrifiers. This study showed that biochar and acetate with appropriate concentration could enhance the enrichment and activity of DAMO bacteria, the results can provide reference for the enrichment of DAMO microbes and its application in the biological nitrogen removal of wastewater. © 2023 Elsevier Ltd
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