Novel near-infrared fluorescence probe with large Stokes shift for monitoring CCl4-induced toxic hepatitis

被引：0

作者：

Tian, Yang ^{[1
]}

Zhou, Dong-Ye ^{[1
]}

Jiang, Wen-Li ^{[1
]}

She, Zun-Pan ^{[1
]}

Li, Yongfei ^{[1
,2
]}

Li, Chun-Yan ^{[1
]}

机构：

[1] Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan,411105, China

[2] College of Chemical Engineering, Xiangtan University, Xiangtan,411105, China

来源：

Talanta | 2021年 / 223卷

基金：

中国国家自然科学基金;

关键词：

Mammals - Health risks - Fluorescence imaging - Infrared devices;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Toxic hepatitis which is induced by chemical substance is a serious threat to human health. More and more studies have shown that peroxynitrite (ONOO−) is related with the development of toxic hepatitis. So it is important to find a tool to study ONOO− change during the diagnosis and therapy of toxic hepatitis. Herein, a series of novel near-infrared (NIR) fluorescence dyes (DDM-R) with long emission wavelength (740770 nm) and large Stokes shift (~200 nm) are developed. Among the dyes, DDM-OH with great spectral performance and facilely modified feature is used to construct probe DDM-ONOO-. The probe have the preference of high sensitivity and excellent selectivity for ONOO−. In addition, DDM-ONOO- was applied in detecting exogenous and endogenous ONOO− in cells and further used in detecting ONOO− of CCl4-induced toxic hepatitis in cells by fluorescence imaging, 3D quantification analysis, flow cytometry. More importantly, by visualizing ONOO−, the probe was used to monitor the diagnosis of CCl4-induced toxic hepatitis in mice and evaluate the therapeutic efficacy of hepatoprotective medicines (NAC, SM, DDB). The results show that the probe will provide a powerful tool for the diagnosis and treatment of toxic hepatitis. © 2020 Elsevier B.V.

引用