Separation, enrichment and cytoprotection of antioxidant peptides from Xuanwei ham using aqueous two-phase extraction

被引：7

作者：

Xie, Rui-hong ^{[1
,3
]}

Xiao, Shan ^{[1
,2
]}

Chen, Xuan ^{[1
,2
]}

Wang, Bo ^{[1
,2
]}

Hu, Yao-yao ^{[1
,3
]}

Wang, Ji-hui ^{[1
,2
,3
]}

机构：

[1] Dongguan Univ Technol, Sch Life & Hlth Technol, Univ Rd,Songshan Lake, Dongguan 523808, Guangdong, Peoples R China

[2] Dongguan Prefabricated Food Innovat Dev & Qual Con, Dongguan 523808, Peoples R China

[3] Dalian Polytech Univ, Coll Biol Engn, Dalian 116034, Peoples R China

来源：

FOOD CHEMISTRY | 2024年 / 446卷

关键词：

Antioxidant peptide; Aqueous two-phase extraction; HaCat cell; Xuanwei ham; Cytoprotection; Peptide screening; ETHANOL/AMMONIUM SULFATE; IDENTIFICATION; PURIFICATION; FRACTIONS; MECHANISM; SYSTEMS; SKIN;

D O I：

10.1016/j.foodchem.2024.138600

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An ethanol/(NH 4 ) 2 SO 4 biphasic (aqueous two-phase) system was designed to effectively separate antioxidant peptides from Xuanwei ham, and its potential to prevent ultraviolet A-induced damage to skin cells was explored. Optimization via single factor experiments and response surface methodology revealed that under 20 % ethanol aqueous solution (w/w), 25.5 % (NH 4 ) 2 SO 4 aqueous solution (w/w), and pH 8.80 conditions, the optimal extraction ratio was 59.0 +/- 1.73 %. In vitro antioxidant activity and cellular assays showed that the peptide purified in the upper phase exhibited strong antioxidant activity, increasing the viability of HaCat cells damaged by UVA irradiation from 56.14 +/- 1.05 % to 66.3 +/- 1.76 %. We used an in silico peptide screening strategy and identified 10 with potential antioxidant activity, emphasizing the important role of amino acids Pro, Gly, and Ala in antioxidant activity.

引用

页数：10

共 39 条

[1] The basal layer in human squamous tumors harbors more UVA than UVB fingerprint mutations: A role for UVA in human skin carcinogenesis [J].