Olive Leaves Extract and Oleuropein Improve Insulin Sensitivity in 3T3-L1 Cells and in High-Fat Diet-Treated Rats via PI3K/AkT Signaling Pathway

被引：17

作者：

Hadrich F. ^{[1
]}

Mahmoudi A. ^{[1
]}

Chamkha M. ^{[1
]}

Isoda H. ^{[2
,3
]}

Sayadi S. ^{[4
]}

机构：

[1] Environmental Bioprocesses Laboratory, Sfax Biotechnology Center, P.O. Box 1177, Sfax

[2] Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Tsukuba

[3] Alliance for Research on Mediterranean and North Africa, University of Tsukuba

[4] Biotechnology Program Center of Sustainable Development, College of Arts and Sciences, Qatar University, Doha

来源：

Oxidative Medicine and Cellular Longevity | 2023年 / 2023卷

关键词：

Cell death - Cell signaling - Histology - Insulin - Polymerase chain reaction - Tissue;

D O I：

10.1155/2023/6828230

中图分类号：

学科分类号：

摘要：

Olive leaves extracts are known to exert potential pharmacological activities especially, antidiabetic and antiobesity. This study explores the anti-insulin resistant effect of olive leaves extracts and oleuropein in 3 T3-L1 cells and in high-fat diet fed rats. Our results showed that ethanol extract (EE) suppressed significantly (P<0.01) triacylglycerol accumulation. In preadipocytes cells, EE 1/100 decreased cell viability and induced apoptosis. Real-time PCR analysis showed that EE reduced the mRNA levels of adipogenesis (CEBP-α, PPARγ, SREBP-1c, and FAS) and proinflammatory (TNF-α and IL-6) genes. Moreover, the cotreatment of EE 1/1000 or oleuropein with insulin increased considerably the expression of p-IRS, p85-pI3K, and p-AKT. In vivo model, the oral administration of oleuropein at 50 mg/kg in rats fed with high fat diet for 8 weeks reduced inflammation in liver and adipose tissues (WAT), improved glucose intolerance, and decreased hyperinsulinemia. Furthermore, the immunohistochemistry revealed that the expression level of p-Akt, IRS1, and Glut-4 were significantly enhanced in liver and WAT tissues after oleuropein supplementation comparing with that in HFD group. Additionally, the expression of IRS1 was markedly ameliorated in pancreas. Our obtained results can be adopted as an approach to used olive leaves as complement to prevent insulin-resistance disease. © 2023 Fatma Hadrich et al.

引用

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