Effect of Heating Temperature on the Structural and Functional Characteristics of Vitexin-Mung Bean Protein

被引:0
作者
Diao J. [1 ,2 ]
Tao Y. [3 ]
Guo H. [3 ]
Chen H. [3 ]
Wang C. [3 ]
Zhang D. [3 ]
Zhu Y. [4 ]
机构
[1] Heilongjiang Bayi Agricultural University, Daqing Center of Inspection and Testing for Agricultural Products, Ministry of Agriculture and Rural Affairs, Heilongjiang, Daqing
[2] National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing
[3] College of Food Science, Heilongjiang Bayi Agricultural University, Heilongjiang, Daqing
[4] Heilongjiang Joyoung Bean Industry Co. Ltd., Heilongjiang, Daqing
关键词
functional properties; heating temperature; mung bean protein; structure; vitexin;
D O I
10.16429/j.1009-7848.2023.08.021
中图分类号
学科分类号
摘要
In order to investigate the structural and functional changes of the complex formed by the interaction of vitexin and mung bean protein (VT-MBP) at different temperatures during mung bean food processing. The effects of heating temperature on the macromolecular structure of vitexin-mungbean protein complexes (VT-MBP) was investigated by Fourier infrared spectroscopy, scanning electron microscopy, fluorescence spectroscopy, and SDS-PAGE. The changes on the functional properties such as solubility, emulsification, foaming, and antioxidant properties of VT-MBP were analyzed under different temperatures. The results showed that: Heating temperature changed the molecular structure of VT-MBP, and the content of a-helix and/3-sheet in the secondary structure of VT-MBP decreased with the increase of temperature. Fluorescence spectroscopic results revealed that the interaction mechanism of VT and MBP was static quenching and binding through non-covalent hydrophobic interaction. In addition, VT-MBP had good emulsification, foaming and foam stability when the heating temperature was 90 °C. Conclusion: The heating temperature has a significant impact on the structure and functionality of VT-MBP. The present results provide a theoretical basis for determining the optimal processing temperature of mung bean food and developing functional mung bean protein beverages. © 2023 Journal of Chinese Institute of Food Science and Technology. All rights reserved.
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页码:197 / 207
页数:10
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