A high-quality genome assembly from short and long reads for the non-biting midge chironomus riparius (Diptera)

被引：0

作者：

Schmidt, Hanno ^{[1
,2
]}

Hellmann, Sören Lukas ^{[3
]}

Waldvogel, Ann-Marie ^{[1
]}

Feldmeyer, Barbara ^{[1
]}

Hankeln, Thomas ^{[3
]}

Pfenninger, Markus ^{[1
,4
]}

机构：

[1] Molecular Ecology Group, Senckenberg Biodiversity and Climate Research Centre, Frankfurt am Main, Hessen, Germany

[2] Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California Davis, CA, United States

[3] Molecular Genetics and Genome Analysis Group, Institute of Organismic and Molecular Evolutionary Biology, Johannes Gutenberg-University, Mainz, Germany

[4] LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt, Germany

来源：

Water (Switzerland) | 2019年 / 11卷 / 09期

关键词：

Molecular biology - Genes - Animals;

D O I：

10.3390/W11091751

中图分类号：

学科分类号：

摘要：

Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius' genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species, and the respective recombination rate among the first insect recombination rates at all. The genome assembly and associated resources will be highly valuable to the broad community working with dipterans in general and chironomids in particular. The estimated recombination rate will help evolutionary biologists gaining a better understanding of commonalities and differences of genomic patterns in insects. © 2020 Schmidt et al.

引用

页码：1151 / 1157