CRISPR Technology for Diagnosis: A Brief Review

Infections and cancers are one of the most serious concerns to humankind, causing a massive needs to be quick, precise, sensitive, and cost-effective. PCR-mediated methods have additional restrictions including extremely specialized equipment, reagents, well-trained staff, and wellestablished facilities. There are three novel CRISPR-mediated methods, including SHERLOCK, DETECTR, and CUT-PCR, developed for precise diagnosis. These new techniques can be attached to other diagnostic methods for achieving more precise strategies. SHERLOCK, DETECTR, and CUT-PCR are potential technologies for the quick detection and diagnosis without complicated processing, however, these are still in the early stage. In the review, we briefly introduced these three CRISPR-mediated diagnostic methods.