Effects of bone surface topography and chemistry on macrophage polarization

被引:13
作者
Ozcolak, Birgun [1 ,2 ]
Erenay, Berkay [1 ]
Odabas, Sedat [3 ,4 ]
Jandt, Klaus D. [5 ]
Garipcan, Bora [1 ]
机构
[1] Bogazici Univ, Inst Biomed Engn, Biomimet & Bioinspired Biomat Res Lab, TR-34684 Istanbul, Turkiye
[2] Istanbul Medipol Univ, Sch Engn & Nat Sci, Dept Biomed Engn, TR-34810 Istanbul, Turkiye
[3] Ankara Univ, Fac Sci, Dept Chem, Biomat & Tissue Engn Lab BteLAB, TR-06560 Ankara, Turkiye
[4] Ankara Univ, Interdisciplinary Res Unit Adv Mat INTRAM, TR-06560 Ankara, Turkiye
[5] Friedrich Schiller Univ Jena, Otto Schott Inst Mat Res, Fac Phys & Astron, Chair Mat Sci CMS, Lobdergraben 32, D-07743 Jena, Germany
来源
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
关键词
Biomimetic; Bone surface topography; Soft lithography; Surface modification osteoimmunomodulation; Macrophages; Macrophage polarization; HUMAN MONOCYTES; COLLAGEN; SCAFFOLDS; HYDROXYAPATITE; CYTOKINES; CELLS; NANO; FABRICATION; OSTEOCLASTOGENESIS; VASCULARIZATION;
D O I
10.1038/s41598-024-62484-3
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Surface structure plays a crucial role in determining cell behavior on biomaterials, influencing cell adhesion, proliferation, differentiation, as well as immune cells and macrophage polarization. While grooves and ridges stimulate M2 polarization and pits and bumps promote M1 polarization, these structures do not accurately mimic the real bone surface. Consequently, the impact of mimicking bone surface topography on macrophage polarization remains unknown. Understanding the synergistic sequential roles of M1 and M2 macrophages in osteoimmunomodulation is crucial for effective bone tissue engineering. Thus, exploring the impact of bone surface microstructure mimicking biomaterials on macrophage polarization is critical. In this study, we aimed to sequentially activate M1 and M2 macrophages using Poly-l-Lactic acid (PLA) membranes with bone surface topographical features mimicked through the soft lithography technique. To mimic the bone surface topography, a bovine femur was used as a model surface, and the membranes were further modified with collagen type-I and hydroxyapatite to mimic the bone surface microenvironment. To determine the effect of these biomaterials on macrophage polarization, we conducted experimental analysis that contained estimating cytokine release profiles and characterizing cell morphology. Our results demonstrated the potential of the hydroxyapatite-deposited bone surface-mimicked PLA membranes to trigger sequential and synergistic M1 and M2 macrophage polarizations, suggesting their ability to achieve osteoimmunomodulatory macrophage polarization for bone tissue engineering applications. Although further experimental studies are required to completely investigate the osteoimmunomodulatory effects of these biomaterials, our results provide valuable insights into the potential advantages of biomaterials that mimic the complex microenvironment of bone surfaces.
引用
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页数:16
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