Transcriptome sequencing and metabolome analysis reveal the molecular mechanism of Salvia miltiorrhiza in response to drought stress

被引:9
作者
Zhou, Ying [1 ,2 ]
Bai, Yan-Hong [2 ]
Han, Feng-Xia [2 ]
Chen, Xue [2 ]
Wu, Fu-Sheng [4 ]
Liu, Qian [2 ,3 ]
Ma, Wen-Zhe [1 ]
Zhang, Yong-Qing [1 ,2 ]
机构
[1] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
[2] Shandong Univ Tradit Chinese Med, Coll Pharm, Jinan, Peoples R China
[3] Minist Educ, Key Lab Tradit Chinese Med Class Theory, Jinan, Peoples R China
[4] Shandong Prov Ctr Forest & Grass, Jinan, Peoples R China
基金
中国国家自然科学基金;
关键词
Salvia miltiorrhiza; Drought stress; Transcriptome; Metabolome; BIOSYNTHESIS; EXPRESSION; GENES; PHARMACOKINETICS; OVEREXPRESSION; TOLERANCE; CHEMISTRY; PATHWAYS; SIGNALS; GROWTH;
D O I
10.1186/s12870-024-05006-7
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Salvia miltiorrhiza is commonly used as a Chinese herbal medicine to treat different cardiovascular and cerebrovascular illnesses due to its active ingredients. Environmental conditions, especially drought stress, can affect the yield and quality of S. miltiorrhiza. However, moderate drought stress could improve the quality of S. miltiorrhiza without significantly reducing the yield, and the mechanism of this initial drought resistance is still unclear. In our study, transcriptome and metabolome analyses of S. miltiorrhiza under different drought treatment groups (CK, A, B, and C groups) were conducted to reveal the basis for its drought tolerance. We discovered that the leaves of S. miltiorrhiza under different drought treatment groups had no obvious shrinkage, and the malondialdehyde (MDA) contents as well as superoxide dismutase (SOD) and peroxidase (POD) activities dramatically increased, indicating that our drought treatment methods were moderate, and the leaves of S. miltiorrhiza began to initiate drought resistance. The morphology of root tissue had no significant change under different drought treatment groups, and the contents of four tanshinones significantly enhanced. In all, 5213, 6611, and 5241 differentially expressed genes (DEGs) were shared in the A, B, and C groups compared with the CK group, respectively. The results of KEGG and co-expression analysis showed that the DEGs involved in plant-pathogen interactions, the MAPK signaling pathway, phenylpropanoid biosynthesis, flavonoid biosynthesis, and plant hormone signal transduction responded to drought stress and were strongly correlated with tanshinone biosynthesis. Furthermore, the results of metabolism analysis indicated that 67, 72, and 92 differentially accumulated metabolites (DAMs), including fumarate, ferulic acid, xanthohumol, and phytocassanes, which were primarily involved in phenylpropanoid biosynthesis, flavonoid biosynthesis, and diterpenoid biosynthesis pathways, were detected in these groups. These discoveries provide valuable information on the molecular mechanisms by which S. miltiorrhiza responds to drought stress and will facilitate the development of drought-resistant and high-quality S. miltiorrhiza production.
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页数:22
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