Haplotype-resolved chromosome-level genome assembly of Ehretia macrophylla

被引：0

作者：

Cheng, Shiping

Zhang, Qikun

Geng, Xining

Xie, Lihua

Chen, Minghui

Jiao, Siqian

Qi, Shuaizheng

Yao, Pengqiang

Lu, Mailin

Zhang, Mengren

Zhai, Wenshan

Yun, Quanzheng

Feng, Shangguo

机构：

[1] Pingdingshan University,Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco

[2] Kaitai-bio Company,economic Woody Plant

[3] Henan Forestry Vocational College,Henan Senzhuang Cukang Agriculture and Forestry Technology Co.

[4] Ltd,College of Life and Environmental Science

[5] Kaitai Mingjing Genetech Corporation,Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants

[6] Hangzhou Normal University,undefined

[7] Hangzhou Normal University,undefined

来源：

SCIENTIFIC DATA | 2024年 / 11卷 / 01期

关键词：

D O I：

10.1038/s41597-024-03791-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ehretia macrophylla Wall, known as wild loquat, is an ecologically, economically, and medicinally significant tree species widely grown in China, Japan, Vietnam, and Nepal. In this study, we have successfully generated a haplotype-resolved chromosome-scale genome assembly of E. macrophylla by integrating PacBio HiFi long-reads, Illumina short-reads, and Hi-C data. The genome assembly consists of two haplotypes, with sizes of 1.82 Gb and 1.58 Gb respectively, and contig N50 lengths of 28.11 Mb and 21.57 Mb correspondingly. Additionally, 99.41% of the assembly was successfully anchored into 40 pseudo-chromosomes. We predicted 58,886 protein-coding genes, of which 99.60% were functionally annotated from databases. We furthermore detected 2.65 Gb repeat sequences, 659,290 rRNAs, 4,931 tRNAs and 4,688 other ncRNAs. The high-quality assembly of the genome offers a solid basis for furthering the fields of molecular breeding and functional genomics of E. macrophylla.

引用

页数：4