The Anti-inflammatory Potential of a Strain of Probiotic Bifidobacterium pseudocatenulatum G7: In Vitro and In Vivo Evidence

被引:12
作者
Sun, Yukun [1 ]
Zhou, Jiazhi [1 ]
Du, Hengjun [1 ]
Zhou, Zhihao [1 ]
Han, Yanhui [1 ,2 ]
Luo, Minna [1 ]
Guo, Xiaojing [1 ]
Gu, Min [1 ]
Yang, Huqing [3 ]
Xiao, Hang [1 ]
机构
[1] Univ Massachusetts, Dept Food Sci, Amherst, MA 01003 USA
[2] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Xian 710119, Peoples R China
[3] Zhejiang Agr & Forest Univ, Coll Food & Hlth, Hangzhou 311300, Peoples R China
基金
美国食品与农业研究所;
关键词
Bifidobacterium pseudocatenulatum; probiotics; inflammatory bowel disease; coliticmice; gut microbiota; bile acids; short-chainfatty acids; BILE-ACID; BIFIDOBACTERIA; MICROBIOTA; METABOLITE; HEALTH;
D O I
10.1021/acs.jafc.3c07935
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The genus Bifidobacterium has been widely used in functional foods for health promotion due to its beneficial effects on human health, especially in the gastrointestinal tract (GIT). In this study, we characterize the anti-inflammatory potential of the probiotic strain Bifidobacterium pseudocatenulatum G7, isolated from a healthy male adult. G7 secretion inhibited inflammatory response in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Moreover, oral administration of bacteria G7 alleviated the severity of colonic inflammation in dextran sulfate sodium (DSS)-treated colitis mice, which was evidenced by a decreased disease activity index (DAI) and enhanced structural integrity of the colon. The 16S rRNA gene sequencing result illustrated that the G7 alleviated DSS-induced gut microbiota dysbiosis, accompanied by the modulated bile acids and short-chain fatty acid (SCFA) levels. Overall, our results demonstrated the potential anti-inflammatory effects of Bifidobacterium pseudocatenulatum G7 on both in vitro and in vivo models, which provided a solid foundation for further development of a novel anti-inflammatory probiotic.
引用
收藏
页码:10355 / 10365
页数:11
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