Contribution of Phenolic Compounds to the Antioxidant Activity of Leaf and Flower Extracts of Sinapis pubescens L. subsp. pubescens (Brassicaceae)

被引：0

作者：

Taviano, Maria Fernanda ^{[1
]}

Arena, Paola ^{[1
,2
]}

Davi, Federica ^{[1
,2
]}

Cavo, Emilia ^{[1
]}

Spadaro, Vivienne ^{[3
]}

Raimondo, Francesco Maria ^{[4
]}

Cacciola, Francesco ^{[5
]}

Vinci, Roberto Lagana ^{[6
]}

Mondello, Luigi ^{[6
,7
]}

Miceli, Natalizia ^{[1
]}

机构：

[1] Univ Messina, Dept Chem BiologicalPharmaceut & Environm Sci, Viale F Stagno Alcontres 31, I-98166 Messina, Italy

[2] Univ Messina, Fdn Prof Antonio Imbesi, Piazza Pugliatti 1, I-98122 Messina, Italy

[3] Univ Palermo, Dept Biol Chem & Pharmaceut Sci & Technol, Sect Bot Anthropol & Zool, Via Archirafi 38, I-90123 Palermo, Italy

[4] PLANTA Res Documentat & Training Ctr, Via Serraglio Vecchio 28, I-90123 Palermo, Italy

[5] Univ Messina, Dept Biomed Dent Morphol & Funct Imaging Sci, Via Consolare Valeria, I-98125 Messina, Italy

[6] Univ Messina, Messina Inst Technol, Former Vet Sch, Dept Chem Biol Pharmaceut & Environm Sci, Viale G Palatucci Snc, I-98168 Messina, Italy

[7] Univ Messina, Former Vet Sch, Dept Chem Biol Pharmaceut & Environm Sci, Chromaleont Srl, Viale G Palatucci Snc, I-98168 Messina, Italy

来源：

CHEMISTRY & BIODIVERSITY | 2024年 / 21卷 / 05期

关键词：

Antioxidants; edible wild plants; polyphenols; phytochemistry; Sicilian vascular flora; MS;

D O I：

10.1002/cbdv.202400272

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within a study focused on Sinapis pubescens subsp. pubescens wild from Sicily (Italy), an edible species still unexplored, our earlier published work has demonstrated good in vitro antioxidant properties for the flower and leaf hydroalcoholic extracts, exhibiting quite different qualitative-quantitative phenolic profiles. Herein, further research was designed to elucidate the role played by phenolic compounds in the different antioxidant mechanisms highlighted for the extracts. To achieve this goal, the crude extracts were subjected to liquid-liquid partitioning with solvents of increasing polarity; then, the fractions were investigated for their antioxidant properties using different in vitro assays. For both flowers and leaves, the ethyl acetate fractions exhibited the best activity in DPPH and reducing power assays, followed by n-butanol. The total phenolic content determination indicated these fractions as the phenolic-rich ones, which were characterized by HPLC-PDA/ESI-MS analysis. Conversely, the phenolic-rich fractions did not show any chelating activity, which was highlighted for the more hydrophobic ones.

引用

页数：10

共 40 条

[1] Phytochemical Characterization of Rhus coriaria L. Extracts by Headspace Solid-Phase Micro Extraction Gas Chromatography, Comprehensive Two-Dimensional Liquid Chromatography, and Antioxidant Activity Evaluation [J].