In planta production of human-derived RANKL

被引：1

作者：

Lee, Jae-Ho ^{[1
]}

Geem, Kyoung Rok ^{[2
]}

Chen, Zhihao ^{[3
]}

Jeong, Jujin ^{[3
]}

Park, Sang-Wook ^{[3
]}

Lee, Dong Wook ^{[1
,2
,4
]}

机构：

[1] Chonnam Natl Univ, Dept Integrat Food Biosci & Biotechnol, Gwangju 61186, South Korea

[2] Chonnam Natl Univ, Dept Bioenergy Sci & Technol, Gwangju 61186, South Korea

[3] Chonnam Natl Univ, Dent Sci Res Inst, Sch Dent, Dept Oral Biochem, Gwangju 61186, South Korea

[4] Chonnam Natl Univ, Bioenergy Res Ctr, Gwangju 61186, South Korea

来源：

PLANT BIOTECHNOLOGY REPORTS | 2024年 / 18卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

RANKL; Nicotiana benthamiana; Molecular farming; Protein expression; Osteoclast differentiation; EXPRESSION; CHLOROPLAST; CELLULASE; PROTEINS;

D O I：

10.1007/s11816-024-00917-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand) is a cytokine that plays a crucial role in bone remodeling by regulating differentiation and activation of osteoclast, which breaks down old bone tissue. In this study, we attempted to produce human-derived RANKL using plant expression systems. The RANKL expressed in Nicotiana benthamiana leaves was designed to accumulate within the endoplasmic reticulum lumen. The purified RANKL from N. benthamiana induced the differentiation of bone marrow-derived macrophages to mature osteoclast, albeit modestly. In conclusion, human-derived RANKL could be produced using a plant expression system, but it requires further improvement.

引用

页码：579 / 585

页数：7

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