Droplet-based cell-laden microgels for high-throughput analysis

被引:2
作者
Li, Xiang [1 ]
Zhao, Danshan [1 ]
Wang, Yuetong [1 ]
Huang, He [1 ]
机构
[1] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210046, Peoples R China
基金
中国国家自然科学基金;
关键词
CYTOMETRY;
D O I
10.1016/j.tibtech.2023.10.010
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cell-laden droplet microfluidics has revolutionized bulk biochemical analysis by offering compartmentalized microreactors for individual cells, but downstream operations of regular aqueous droplets are limited. Hydrogel matrix can provide a rigid scaffold for long -term culture of eukaryotic and prokaryotic cells, and can support several manipulations, facilitating subsequent high-throughput analysis of cellular heterogeneity.
引用
收藏
页码:397 / 401
页数:5
相关论文
共 15 条
  • [1] Suspendable Hydrogel Nanovials for Massively Parallel Single-Cell Functional Analysis and Sorting
    de Rutte, Joseph
    Dimatteo, Robert
    Archang, Maani M.
    van Zee, Mark
    Koo, Doyeon
    Lee, Sohyung
    Sharrow, Allison C.
    Krohl, Patrick J.
    Mellody, Michael
    Zhu, Sheldon
    Eichenbaum, James, V
    Kizerwetter, Monika
    Udani, Shreya
    Ha, Kyung
    Willson, Richard C.
    Bertozzi, Andrea L.
    Spangler, Jamie B.
    Damoiseaux, Robert
    Di Carlo, Dino
    [J]. ACS NANO, 2022, 16 (05) : 7242 - 7257
  • [2] Flow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungi
    Delgado-Ramos, Lidia
    Marcos, Ana T.
    Ramos-Guelfo, Maria S.
    Sanchez-Barrionuevo, Leyre
    Smet, Francis
    Chavez, Sebastian
    Canovas, David
    [J]. G3-GENES GENOMES GENETICS, 2014, 4 (11): : 2271 - 2278
  • [3] A Gelatin Microdroplet Platform for High-Throughput Sorting of Hyperproducing Single-Cell-Derived Microalgal Clones
    Li, Ming
    van Zee, Mark
    Riche, Carson T.
    Tofig, Bobby
    Gallaher, Sean D.
    Merchant, Sabeeha S.
    Damoiseaux, Robert
    Goda, Keisuke
    Di Carlo, Dino
    [J]. SMALL, 2018, 14 (44)
  • [4] Gel microdroplet-based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris
    Ma, Chunxuan
    Tan, Zheng Lin
    Lin, Ying
    Han, Shuangyan
    Xing, Xinhui
    Zhang, Chong
    [J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2019, 128 (06) : 662 - 668
  • [5] Microfluidics-based fabrication of cell-laden microgels
    Mohamed, Mohamed G. A.
    Ambhorkar, Pranav
    Samanipour, Roya
    Yang, Annie
    Ghafoor, Ali
    Kim, Keekyoung
    [J]. BIOMICROFLUIDICS, 2020, 14 (02)
  • [6] High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads
    Napiorkowska, Marta
    Pestalozzi, Luzius
    Panke, Sven
    Held, Martin
    Schmitt, Steven
    [J]. SMALL, 2021, 17 (02)
  • [7] Multiscale cytometry and regulation of 3D cell cultures on a chip
    Sart, Sebastien
    Tomasi, Raphael F. -X.
    Amselem, Gabriel
    Baroud, Charles N.
    [J]. NATURE COMMUNICATIONS, 2017, 8
  • [8] Single gene-based distinction of individual microbial genomes from a mixed population of microbial cells
    Tamminen, Manu V.
    Virta, Marko P. J.
    [J]. FRONTIERS IN MICROBIOLOGY, 2015, 6
  • [9] Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture
    Utech, Stefanie
    Prodanovic, Radivoje
    Mao, Angelo S.
    Ostafe, Raluca
    Mooney, David J.
    Weitz, David A.
    [J]. ADVANCED HEALTHCARE MATERIALS, 2015, 4 (11) : 1628 - 1633
  • [10] High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering
    Vanella, Rosario
    Kovacevic, Gordana
    Doffini, Vanni
    de Santaella, Jaime Fernandez
    Nash, Michael A.
    [J]. CHEMICAL COMMUNICATIONS, 2022, 58 (15) : 2455 - 2467