Tumor microenvironment-responsive gold nanodendrites for nanoprobe-based single-cell Raman imaging and tumor-targeted chemo-photothermal therapy

被引：0

作者：

Shuai Y. ^{[1
]}

Bao Q. ^{[2
]}

Yue H. ^{[2
]}

Wang J. ^{[1
]}

Yang T. ^{[2
]}

Wan Q. ^{[1
]}

Zhong Y. ^{[2
]}

Xu Z. ^{[1
]}

Mao C. ^{[2
,3
]}

Yang M. ^{[1
]}

机构：

[1] Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou

[2] School of Materials Science and Engineering, Zhejiang University, Hangzhou

[3] Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, Sha Tin

来源：

Smart Materials in Medicine | 2023年 / 4卷

关键词：

Gold nanoparticles; Mild photothermal heating; Multimodal bioimaging; SERS imaging; Silk fibroin; Targeted therapy;

D O I：

10.1016/j.smaim.2023.06.002

中图分类号：

学科分类号：

摘要：

Nanodendrite particles (NDs) with densely branched structures and biomimetic architectures have exhibited great promise in tumor therapy owing to their prolonged in vivo circulation time and exceptional photothermal efficiency. Nevertheless, traditional NDs are deficient in terms of specific surface modification and targeting tumors, which restrict their potential for broader clinical applications. Here, we developed coronavirus-like gold NDs through a seed-mediated approach and using silk fibroin (SF) as a capping agent. Our results demonstrate that these NDs have a favorable drug-loading capacity (∼65.25%) and light-triggered release characteristics of doxorubicin hydrochloride (DOX). Additionally, NDs functionalized with specific probes exhibited exceptional surface-enhanced Raman scattering (SERS) characteristics, enabling high-sensitivity Raman imaging of unstained single cells. Moreover, these NDs allowed for real-time monitoring of endocytic NDs for over 24 h. Furthermore, ND@DOX conjugated with tumor-targeting peptides exhibited mild hyperthermia, minimal cytotoxicity, and effective targeting towards cancer cells in vitro, as well as responsiveness to the tumor microenvironment (TME) in vivo. These unique properties led to the highest level of synergistic tumor-killing efficiency when stimulated by a near-infrared (NIR) laser at 808 nm. Therefore, our virus-like ND functionalized with SF presents a novel type of nanocarrier that exhibits significant potential for synergistic applications in precision medicine. © 2023 The Authors

引用

页码：680 / 689

页数：9