Red blood cells membrane micropolarity as a novel diagnostic indicator of type 1 and type 2 diabetes

被引：9
作者：
Bianchetti G. [1 ,2 ]
Di Giacinto F. [1 ,2 ]
Pitocco D. [1 ,3 ]
Rizzi A. [1 ,3 ]
Rizzo G.E. [1 ,3 ]
De Leva F. [1 ,3 ]
Flex A. [1 ,4 ]
di Stasio E. [1 ,5 ]
Ciasca G. [1 ,2 ]
De Spirito M. [1 ,2 ]
Maulucci G. [1 ,2 ]
机构：
[1] Fondazione Policlinico Universitario A, Gemelli IRCSS, Rome
[2] Istituto di Fisica, Università Cattolica Del Sacro Cuore, Rome
[3] Diabetes Care Unit, Università Cattolica Del Sacro Cuore, Rome
[4] Cardiovascular Disease Division, Università Cattolica Del Sacro Cuore, Rome
[5] Istituto di Biochimica Clinica, Università Cattolica Del Sacro Cuore, Rome
来源：
Analytica Chimica Acta: X | 2019年 / 3卷
关键词：
Diabetes mellitus; Fluorescence lifetime microscopy; Membrane micropolarity; Metabolic imaging; Personalized medicine; Red blood cells;
D O I：
10.1016/j.acax.2019.100030
中图分类号：
学科分类号：
摘要：
Classification of the category of diabetes is extremely important for clinicians to diagnose and select the correct treatment plan. Glycosylation, oxidation and other post-translational modifications of membrane and transmembrane proteins, as well as impairment in cholesterol homeostasis, can alter lipid density, packing, and interactions of Red blood cells (RBC) plasma membranes in type 1 and type 2 diabetes, thus varying their membrane micropolarity. This can be estimated, at a submicrometric scale, by determining the membrane relative permittivity, which is the factor by which the electric field between the charges is decreased relative to vacuum. Here, we employed a membrane micropolarity sensitive probe to monitor variations in red blood cells of healthy subjects (n=16) and patients affected by type 1 (T1DM, n=10) and type 2 diabetes mellitus (T2DM, n=24) to provide a cost-effective and supplementary indicator for diabetes classification. We find a less polar membrane microenvironment in T2DM patients, and a more polar membrane microenvironment in T1DM patients compared to control healthy patients. The differences in micropolarity are statistically significant among the three groups (p<0.01). The role of serum cholesterol pool in determining these differences was investigated, and other factors potentially altering the response of the probe were considered in view of developing a clinical assay based on RBC membrane micropolarity. These preliminary data pave the way for the development of an innovative assay which could become a tool for diagnosis and progression monitoring of type 1 and type 2 diabetes. © 2019 The Authors
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