High rate 2,3-butanediol production with Vibrio natriegens

被引：30

作者：

Erian A.M. ^{[1
]}

Freitag P. ^{[1
]}

Gibisch M. ^{[1
]}

Pflügl S. ^{[1
]}

机构：

[1] Technische Universität Wien, Institute for Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Gumpendorfer Straße 1a, Vienna

来源：

Bioresource Technology Reports | 2020年 / 10卷

关键词：

2,3-Butanediol; Chemostat cultivations; Elemental biomass composition; High productivity; Microaerobic conditions; Vibrio natriegens;

D O I：

10.1016/j.biteb.2020.100408

中图分类号：

学科分类号：

摘要：

The aim of this study was to investigate if the high substrate turnover rates of Vibrio natriegens enable 2,3-butanediol production at high productivities. Introduction of a heterologous pathway led to 2,3-butanediol production at yields comparable to Escherichia coli using glucose, sucrose and sugar beet molasses. Microaerobic fed-batch cultivations maintained at 0–1% dissolved oxygen yielded a combined diol titer of 49.9 g L−1 of 2,3-butanediol and acetoin and a production rate of 3.9 g L−1 h−1. Optimization of the oxygen supply in chemostat cultivations increased the product yield to 70% of the theoretical maximum at volumetric and specific production rates of 2 g L−1 h−1 and 0.66 g g−1 h−1, respectively. In conclusion, this study could for the first time demonstrate V. natriegens as novel host for 2,3-butanediol production with high volumetric and specific productivity, underlining its potential for microbial chemical production. © 2020 The Author(s)

引用

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