Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans

被引：0

作者：

Zhuang Y. ^{[1
]}

Yang F. ^{[1
]}

Xiao M. ^{[1
]}

Tian X. ^{[1
]}

Chen Y. ^{[1
]}

Long Z. ^{[1
]}

Chen B. ^{[1
]}

Ni Y. ^{[2
]}

Gong A. ^{[1
]}

Xie Y. ^{[1
]}

机构：

[1] College of Traditional Chinese Medicine, Hainan Medical College, Haikou

[2] Department of Pharmacy, The Second People's Hospital of Hainan Province, Haikou

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 11期

关键词：

anti-aging; antioxidant; Caenorhabditis elegans; Clinacanthus nutans ethanol extract; daf-16;

D O I：

10.13386/j.issn1002-0306.2022090024

中图分类号：

学科分类号：

摘要：

Objective: To assess the anti-aging effect and mechanism underlying the effects of ethanol extract from Clinacanthus nutans (CNEE) using Caenorhabditis elegans as the model organism. Methods: The anti-aging effects of ethanol extract from Clinacanthus nutans on nematodes were detected by experiments to assay longevity, oviposition, and exercise. The antioxidant capacity of the ethanolic extracts from Clinacanthus nutans and their mechanisms of action in delaying aging were investigated by assessing the acute oxidative stress, determining the antioxidant enzyme activity, and determining the reactive oxygen species (ROS) levels along with real-time fluorescence quantitative PCR experiments. Results: The ethanol extract from Clinacanthus nutans at 100 μg/mL could prolong the life span of N2 wild type nematode (P<0.001), promote the ability to exercise (P<0.001), and exerted no toxic effects on oviposition (P>0.05). The extract also increased nematode longevity (P<0.05) and catalase activity (P<0.01) under acute oxidative stress conditions, and effectively reduced the accumulation of ROS in vivo (P<0.05) along with upregulating the levels of daf-16 (P<0.01) and downstream ctl-1 (P<0.001) gene expressions. However, in daf-16 mutant nematodes, effects on life extension under normal conditions or acute oxidative stress were not observed, nor was there an increase in catalase activity, a decrease in ROS levels, or an increase in daf-16 and ctl-1 levels (P>0.05). Conclusion: The ethanol extract from Clinacanthus nutans enhanced the ability of nematodes to resist aging and the underlying mechanism of action may be related to the regulation of oxidative stress by daf-16. © 2023 Science and Technology of Food Industry. All rights reserved.

引用

页码：411 / 417

页数：6

共 33 条

[1]

LIU H N., Preparation of hydrogen-rich water and the mechanism of Clostridium butyricum to prolong the life span of Caenorhabditis elegans, (2020)

[2]

HUANG X B., Screening of anti-aging compounds and evaluation of anti-aging activity of aspirin, (2016)

[3]

GUO X Y, LI Q Q, SHI J, Et al., Perfluorooctane sulfonate exposure causes gonadal developmental toxicity in Caenorhabditis elegans through ROS-induced DNA damage[J], Chemosphere, 155, (2016)

[4]

SHAY J W., Role of telomeres and telomerase in aging and cancer[J], Cancer Discovery, 6, 6, (2016)

[5]

DUAN J H., Study on the anti-drinking, hepatoprotective and antioxidant functions of the leaf extracts of Clinacanthus nutans, (2019)

[6]

WANG Y, ZHONG X W, ZHANG W X., Advances in the chemical composition and pharmacological effects of Clinacanthus nutans[J], Chinese Pharmacy, 27, 1, (2016)

[7]

WEI R., Extraction, purification and functional evaluation of triterpenoids from Clinacanthus nutans, (2020)

[8]

DUAN J H, XU D P, QIAN Z J, Et al., Study on the antioxidant efficacy and composition of Clinacanthus nutans[J], Food Science and Technology, 44, 3, pp. 205-210, (2019)

[9]

HUANG Z, LI J, DU H Z, Et al., Lipid lowering effect of Panax notoginseng alcohol extract on Caenorhabditis elegans[J], World Science and Technology-Modernization of Traditional Chinese Medicine, 22, 5, pp. 1648-1653, (2020)

[10]

LIU J., Study on the anti aging effect and mechanism of total alkaloids of Dendrobium nobile on Caenorhabditis elegans, (2019)

← 1 2 3 4 →