Screening of a lignin degrading bacterium and its degradation pathway

被引：0
|
作者：
Wang J. [1 ]
Ni J. [1 ]
Wang L. [1 ]
Qing Q. [1 ]
Yan S. [1 ]
Zhang Y. [1 ]
机构：
[1] School of Pharmacy, Changzhou University, Changzhou
来源：
Huagong Jinzhan/Chemical Industry and Engineering Progress | 2021年 / 40卷 / 07期
关键词：
Degradation pathway; Lignin; Lignin peroxidase; Manganese peroxidase;
D O I：
10.16085/j.issn.1000-6613.2020-1541
中图分类号：
学科分类号：
摘要：
The content of lignin and alkaline salt in the pretreatment solution of corn straw obtained by high temperature alkaline salt treatment was high. In order to reuse the pretreatment solution containing salt, soluble lignin should be removed. In this experiment, a lignin-degrading strain CCZU-WJ6 was screened and isolated from rotten wood and was identified as Achromobacter sp. by morphological observation and 16S rDNA sequence analysis. The activities of enzymes related to lignin degradation in CCZU-WJ6, the growth trend of CCZU-WJ6 in corn straw pretreatment solution, COD removal rate and lignin degradation rate of the pretreatment solution were measured. GC-MS and FTIR were used to detect the changes of components and chemical bonds in the pretreatment solution. Through single-factor experiment, the optimal conditions for degradation of the pretreatment solution by CCZU-WJ6 were determined as pH 7.0, temperature 30℃, dilution factor 10 times, and inoculation amount 0.5g/mL. After 6 days of treatment, the removal rate of COD and the degradation rate of lignin were 40.9% and 32.1%, respectively. The activities of lignin peroxidase and manganese peroxidase secreted by CCZU-WJ6 were 215.5U/L and 200.1U/L, respectively. CCZU-WJ6 can destroy the benzene ring structure, ether bond and C=O bond in the lignin structure, and the degradation products contained guaiacol and p-coumaric acid, which inferred that the degradation pathways are β-aryl ether metabolic pathway and ferulic acid metabolic pathway. CCZU-WJ6 can be used to degrade industrial wastewater containing lignin and has broad prospects in industrial applications. © 2021, Chemical Industry Press Co., Ltd. All right reserved.
引用
收藏
页码：4021 / 4026
页数：5
相关论文
共 13 条
  • [1] LOPES A M C, GOAO K G, RUBIK D F, Et al., Pre-treatment of lignocellulosic biomass using ionic liquids: wheat straw fractionation, Bioresource Technology, 142, pp. 198-208, (2013)
  • [2] GREGOROVA A, CIBULKOVA Z, KOSIKOVA B, Et al., Stabilization effect of lignin in polypropylene and recycled polypropylene, Polymer Degradation and Stability, 89, 3, pp. 553-558, (2005)
  • [3] CARDOSO M, OLIVEIRA E D D, PASSOS M L., Chemical composition and physical properties of black liquors and their effects on liquor recovery operation in Brazilian pulp mills, Fuel, 88, 4, pp. 756-763, (2009)
  • [4] FONSECA M I, FARINA J I, CASTRILLO M L, Et al., Biopulping of wood chips with Phlebia brevispora BAFC 633 reduces lignin content and improves pulp quality, International Biodeterioration & Biodegradation, 90, pp. 29-35, (2014)
  • [5] CHEN Yuehui, CHAI Liyuan, TANG Chongjian, Et al., Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process, Bioresource Technology, 123, pp. 682-685, (2012)
  • [6] JOY S, RAHMAN P K S M, SHARMA S., Biosurfactant production and concomitant hydrocarbon degration potentials of bacteria isolated from extreme and hydrocarbon contaminated environments, Chemical Engineering Journal, 317, pp. 232-241, (2017)
  • [7] TABASSUM S, JI Qinhong, HENA S, Et al., Treatment of coal gasification wastewater by anaerobic SBR-aerobic SBR process for elimination of toxic organic matters-A lab scale study, RSC Advances, 5, 72, pp. 58334-58344, (2015)
  • [8] LARA M A, RODRI GUEZ-MALAVER A J, ROJAS O J, Et al., Black liquor lignin biodegradation by Trametes elegans, International Biodeterioration & Biodegradation, 52, 3, pp. 167-173, (2003)
  • [9] SU Yulong, Screening and characteristics of high-efficiency degrading tobacco stem strains, (2016)
  • [10] CHEN Y H, CHAI L Y, ZHU Y H, Et al., Biodegradation of kraft lignin by a bacterial strain Comamonas sp. B-9 isolated from eroded bamboo slips, Journal of Applied Microbiology, 112, 5, pp. 900-906, (2012)
12