Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C

被引：1

作者：

Garriga, Ferran ^{[1
,2
]}

Maside, Carolina ^{[1
,2
]}

Padilla, Lorena ^{[1
,2
]}

Recuero, Sandra ^{[1
,2
]}

Rodriguez-Gil, Joan E. ^{[3
]}

Yeste, Marc ^{[1
,2
,4
]}

机构：

[1] Univ Girona, Inst Food & Agr Technol, Biotechnol Anim & Human Reprod TechnoSperm, Girona 17003, Spain

[2] Univ Girona, Fac Sci, Dept Biol, Unit Cell Biol, Girona 17003, Spain

[3] Autonomous Univ Barcelona, Fac Vet Med, Dept Anim Med & Surg, Unit Anim Reprod, Barcelona 08193, Spain

[4] Catalan Inst Res & Adv Studies ICREA, Barcelona 08010, Spain

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

HSP70; YM-1; Sperm; Liquid storage; Flow cytometry; Pig; IN-VITRO CAPACITATION; BOAR SPERM; ARTIFICIAL-INSEMINATION; SEMEN QUALITY; SUBPOPULATIONS; MOTILITY; SPERMATOZOA; APOPTOSIS; PRESERVATION; ASSOCIATION;

D O I：

10.1038/s41598-024-64488-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

At present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 degrees C. Six semen pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 mu M), an HSP70 inhibitor, and stored at 17 degrees C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca2+ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function.

引用

页数：11