Sustainable Synthesis of CuO Nanoparticles for Colorimetric Detection of Hydrogen Peroxide and Glucose

The current study demonstrates an easy utilization of glycine soja sensu auct green extract as a power source for the desired synthesis of copper oxide (CuO) nanoparticles via green synthesis process to acquire significant detection of hydrogen peroxide (H2O2) and monosaccharide glucose through an uncomplicated colorimetric method. Due to sustainability issues, synthesis of the metal or metal oxide nanoparticles has shifted towards eco-safe reducing agents. This research focuses on the utilization of the reducing agent via green technology, namely glycine soja sensu auct extract, for the synthesis of CuO nanoparticles by co-precipitation synthesis. Through XRD studies, it can be depicted that the material is monoclinic. FESEM and HRTEM images reveals spherical shape of nanoparticles. HRTEM images also indicate that the size of the particles falls between 10-30 nm. The particle distribution curve reveals the z-average size of 135.1 nm. The Fourier transform infrared spectroscopy peak at 493.14, 522.01, 557.89 and 649.39 cm-1 refer to the vibrations between Cu and O. Clearly, the peaks indicate the formation of CuO nanostructure. These green CuO NPs successfully catalyzed the oxidation reaction of the exposed peroxidase substrate 3, 3 ', 5, 5 '- tetramethylbenzidine (TMB) dye by hydrogen peroxide (H2O2) to develop a bluish colour reaction with the absorption maxima centred at 652 nm. The CuO NPs exhibited better kinetics over horseradish peroxidase (HRP), the natural enzyme, with a lower Km value. Linear response range for glucose, was 10 - 100 mu M and a limit of detection (5.06 mu M) was obtained.