Method to detect virulence gene rfbE of Escherichia coli O157:H7 in textiles

被引：0

作者：

Li K. ^{[1
]}

Zhang Z. ^{[1
]}

Yu J. ^{[1
]}

Lian S. ^{[2
]}

Ding Y. ^{[3
]}

Xie T. ^{[4
]}

Fu K. ^{[5
]}

Guo H. ^{[1
]}

机构：

[1] Zhengzhou Customs, Zhengzhou

[2] Shijiazhuang Customs, Shijiazhuang

[3] Nanjing Customs Industrial Products Testing Center, Nanjing

[4] Shenzhen Customs Industrial Products Testing Technology Center, Shenzhen

[5] Ningbo Institute of Inspection Science and Technology, Ningbo

来源：

Fangzhi Xuebao/Journal of Textile Research | 2021年 / 42卷 / 10期

关键词：

Escherichia coli O157:H7; Immunomagnetic bead; Real-time PCR; Textile; Virulence gene;

D O I：

10.13475/j.fzxb.20201101207

中图分类号：

学科分类号：

摘要：

In order to establish a sensitive, specific, rapid and efficient method to evaluate E. coli O157:H7 in textiles, the conservative rfb E sequence of E. coli O157:H7 was screened for the design of PCR specific primers and fluorescent double labeled probes. Combined with immunomagnetic beads technology, a new technology was developed for quick and efficient isolation of E. coli O157:H7 under the condition of low concentration and non-culturable target bacteria. A real-time fluorescent quantitative PCR method was developed, and the detection limit could reach 8 CFU/g. 30 positive samples were identified using this method, and the results were 100% consistent with the results from using the traditional methods. The results show that the new method is stable, rapid and sensitive in identifying E. coli O157:H7 in textiles. © 2021, Periodical Agency of Journal of Textile Research. All right reserved.

引用

页码：92 / 98

页数：6

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