Raman spectroscopy-based molecularly imprinted polymer sensor for sensitive detection of lysophosphatidic acid in serum

Lysophosphatidic acid (LPA) has significant potential as a biomarker for diagnosis of ovarian cancer in female patients because it is present throughout all phases of the illness. Regrettably, there is not a clinically useful test for this biomarker yet. In this work, an ultra-detection of LPA molecule by Raman spectroscopy-based molecularly imprinted polymer (MIP) in serum samples is reported. beta-CD was synthesized to facilitate the collection of Raman spectroscopy and combined with MIP to denote it as beta-CD-MIP for the adsorption of LPA with high sensitivity and selectivity. Linear regression model in serum samples shows peaks at 1100 and 1116 cm-1, respectively. A satisfactory linear fit was seen with LPA concentration log I = 3.7 log C + 1255.74 and with spiked serum log I = 4.6 log C + 327.22, respectively. The literature survey suggests the threshold LPA level in healthy person is 1.50 mu M/L whereas the LPA level rises in patients with benign ovarian cancer tumor as 7.90 mu M/L. This LPA level rises to 16.99 mu M/L in ovarian cancer patients. On the basis of the band intensity, the limit of detection of the beta-CD-MIP sensor for LPA was recorded as 0.003 mu M/L. The binding constant of beta-CD-MIP for LPA in serum was 0.031-0.003 mu M/L, respectively. beta-CD-MIP gel was optimized, characterized, and successfully applied on spiked serum samples. The findings of this research show that this substrate has a lot of potential for ultradetection of trace amounts of LPA molecule from the serum samples. The MIP sensor is robust, recyclable, efficient, and selective. Molecular imprinting technique is used to develop an imprinted beta-CD polymer using LPA as template. This MIP prepared was imprinted with LPA cavity, which has been extended for Raman spectroscopy. MIP is prepared from adduct by washing LPA template with solvent. The LPA is removed and a particular cavity of a template is imprinted in a polymer. The cavity is complementary to the shape and size of the template molecule (LPA). The capacity of developed MIPs to detect LPA in serum samples that have been spiked is also observed. image