Effects of Storage Time of Raw Beef on Microflora Changes and Protein Degradation in Fermented Beef Sausages

被引：0

作者：

Xu Y. ^{[1
]}

Liu S. ^{[1
]}

Wang Y. ^{[1
]}

Niu S. ^{[1
]}

Yang Y. ^{[1
]}

Yu Q. ^{[1
]}

Xiao Z. ^{[1
]}

Liu S. ^{[1
]}

He L. ^{[1
]}

Chen S. ^{[1
]}

Liu A. ^{[1
]}

Yang Y. ^{[1
]}

机构：

[1] College of Food Science, Sichuan Agricultural University, Ya'an

来源：

Shipin Kexue/Food Science | 2021年 / 42卷 / 09期

关键词：

Biogenic amine; Fermented beef sausage; Microorganism; Nitrogen-containing compound;

D O I：

10.7506/spkx1002-6630-20200414-183

中图分类号：

学科分类号：

摘要：

Traditional microbial counting methods, high-throughput sequencing, the Kjeldahl method and high performance liquid chromatography (HPLC) were applied to explore the effects of different postmortem storage times of beef (0, 12, 24 and 36 h at 0-4℃) on microflora changes and proteolysis during the processing of fermented beef sausages as well as on the correlation between them. The results showed that the total number of bacterial colonies, coliform count, the diversity and richness of bacterial communities and proteolysis indexes increased, while the content of total free amino acids in fermented beef sausages decreased as the storage time of raw beef increased. A significant positive correlation appeared between total volatile basic nitrogen (TVB-N) and total biogenic amine contents during the ripening process (P < 0.01). The average values of total number of bacterial colonies and Shannon diversity index of fermented sausage prepared from 36 h-chilled beef were 4.03 × 107 CFU/g and 2.89, respectively, which were both higher than those (2.40 × 107 CFU/g and 1.39) of fermented sausage prepared from unchilled fresh beef (P < 0.05 for total number of bacterial colonies). The dominant bacteria in the former were Staphylococcus over the whole processing period, while the dominant bacteria in the latter were Weissella spp. at the early stage and Staphylococcus at the late stage. The average total free amino acid content decreased from 7.09 to 6.44 mg/g, whereas the average total biogenic amine content increased from 92.14 to 117.42 mg/kg (P < 0.05). These results indicated that controlling the storage time of raw beef could effectively reduce the total microbial load and the microbial diversity of fermented sausages, retard the accumulation of biogenic amines and inhibit proteolysis, thereby ensuring the safety of fermented sausages. © 2021, China Food Publishing Company. All right reserved.

引用

页码：184 / 191

页数：7

共 36 条

[1] pp. 1-2, (2013)
[2] RABIE M A, PERES C, MALCATA F X, Et al., Evolution of amino acids and biogenic amines throughout storage in sausages made of horse, beef and turkey meats, Meat Science, 96, 1, pp. 82-87, (2014)
[3] STADNIK J, DOLATOWSKI Z J., Biogenic amines in meat and fermented meat products, Acta Scientiarum Polonorum: Technologia Alimentaria, 9, 3, pp. 251-263, (2010)
[4] WANG X H, REN H Y, WANG W, Et al., Evaluation of key factors influencing histamine formation and accumulation in fermented sausages, Journal of Food Safety, 35, 3, pp. 395-402, (2015)
[5] IKONIC P M, TASIC T A, PETROVIC L S, Et al., Drying characteristics of traditional fermented sausage Petrovska klobasa: The effect of different ripening conditions and use of starter culture, Food and Feed Research, 41, 1, pp. 71-79, (2014)
[6] SETTANNI L, MOSCHETTI G., New trends in technology and identity of traditional dairy and fermented meat production processes: Preservation of typicality and hygiene, Trends in Food Science and Technology, 37, 1, pp. 51-58, (2014)
[7] COCOLIN L, DOLCI P, RANTSIOU K, Et al., Biodiversity and dynamics of meat fermentations: The contribution of molecular methods for a better comprehension of a complex ecosystem, Meat Science, 89, 3, pp. 296-302, (2011)
[8] PARKS G S, OLIVAS N D, IKRAR T, Et al., Histamine inhibits the melanin-concentrating hormone system: Implications for sleep and arousal, The Journal of Physiology, 592, 10, pp. 2183-2196, (2014)
[9] TOSUKHOWONG A, VISESSANGUAN W, PUMPUANG M, Et al., Biogenic amine formation in Nham a Thai fermented sausage, and the reduction by commercial starter culture, Lactobacillus plantarum BCC 9546, Food Chemistry, 129, 3, pp. 846-853, (2011)
[10] pp. 1-4, (2016)

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