Immunomodulatory Effect of Polysaccharide Extracted from Sanghuangporus baumi Mycelia in LPS-Induced RAW264.7 Macrophages

被引：0
作者：
Ma X. [1 ]
Xiao J. [1 ]
Gong N. [1 ]
Liu G. [1 ]
Chen X. [1 ]
Yang T. [1 ]
Yao L. [2 ]
Li C. [2 ]
Zhao Y. [1 ]
机构：
[1] Liaoning Academy of Agricultural Sciences, Edible Fungus Institute, Key Laboratory of Edible Fungi High Quality Cultivation, Shenyang
[2] Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun
来源：
Science and Technology of Food Industry | 2023年 / 44卷 / 20期
关键词：
cytokines; lipopolysaccharide; macrophages; Sanghuangporus baumi;
D O I：
10.13386/j.issn1002-0306.2022120011
中图分类号：
学科分类号：
摘要：
Sanghuangporus baumi is a kind of medicinal fungus with high activity. The aim of the present study was to investigate the immunoregulatory effects of Sanghuangporus baumi mycelia polysaccharides (SJ) on lipopolysaccharide (LPS)-induced RAW264.7 cells. In this study, Sanghuangporus baumi mycelia polysaccharides (SJ) were taken as the study subjects，and LPS-stimulated RAW264.7 macrophages were used as the cell model. The experiment was divided into three groups: Control group, LPS (model) and LPS+SJ groups. A CCK-8 kit was used to detect the viability of RAW264.7 cells. The effects of SJ on nitric oxide, and cytokines (IL-6, IL-1β, and TNF-α) production in LPS-stimulated RAW264.7 macrophages were measured by enzyme-linked immunoassay assay (ELISA). Furthermore, the effects of SJ on the protein levels of IL-6, TNF-α, IL-1β and inducible nitric oxide synthase (iNOS) were investigated by Western-Blot analysis. The results indicated that SJ increased 20.5%~50% cell viability at different concentrations compared to the control group. SJ at 200 μg/mL potently inhibited the release of nitric oxide and cytokine production in LPS-stimulated RAW264.7 (P<0.001), and compared with the LPS group, the secretion of NO, TNF-α, IL-6 and IL-1β decreased by 21.62%, 29.99%, 35.5% and 50.03%, respectively. Moreover, SJ also reduced LPS-induced protein expression levels of IL-6, TNF-α, IL-1β and iNOS in a dose-dependent fashion (P<0.001), and alleviated the inflammatory damage induced by LPS. And it was consistent with the change of cytokine secretion detected by ELISA method. The results of the present study suggest that Sanghuangporus baumi mycelia polysaccharides (SJ) have good immunomodulatory. © 2023 Science and Technology of Food Industry. All rights reserved.
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页码：407 / 413
页数：6
相关论文
共 28 条
  • [1] HEROLD K, MROWKA R., Inflammation-dysregulated inflammatory response and strategies for treatment[J], Acta Physiol (Oxf), 226, 3, (2019)
  • [2] LUAN F, PENG X, ZHAO G, Et al., Structural diversity and bioactivity of polysaccharides from medicinal mushroom Phellinus spp.: A review[J], Food Chem, 397, (2022)
  • [3] WANG H, MA J X, ZHOU M, Et al., Current advances and potential trends of the polysaccharides derived from medicinal mushrooms Sanghuang[J], Front Microbiol, 13, (2022)
  • [4] AJITH T., Antidiabetic properties of medicinal mushrooms with special reference to Phellinus species: A review[J], The Natural Products Journal, 11, pp. 120-126, (2021)
  • [5] ZHANG J J, CHEN B S, DAI H Q, Et al., Sesquiterpenes and polyphenols with glucose-uptake stimulatory and antioxidant activities from the medicinal mushroom Sanghuangporus Sanghuang[J], Chin J Nat Med, 19, 9, pp. 693-699, (2021)
  • [6] HE P, ZHANG Y, LI N., The phytochemistry and pharmacology of medicinal fungi of the genus Phellinus: A review[J], Food Funct, 12, 5, pp. 1856-1881, (2021)
  • [7] JHY A, YSLA B, SKK C, Et al., Phellinus baumii enhances the immune response in cyclophosphamide-induced immunosuppressed mice[J], Nutrition Research, 75, pp. 15-31, (2020)
  • [8] YS A, JH A, SHI Z A, Et al., Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii[J], Carbohydrate Polymers, 268, (2021)
  • [9] ZUO K, TANG K, LIANG Y, Et al., Purification and antioxidant and anti-inflammatory activity of extracellular polysaccharopeptide from sanghuang mushroom, Sanghuangporus lonicericola[J], J Sci Food Agric, 101, 3, (2021)
  • [10] WANG Z, ZHOU F, QUAN Y., Antioxidant and immunological activity in vitro of polysaccharides from Phellinus nigricans mycelia[J], Int J Biol Macromol, 64, pp. 139-143, (2014)
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